The MNase Advantage
The MNase enzyme possesses both sequence-independent endonuclease and exonuclease activities to generated uniform fragments that are nucleosome length (146 bp). This overcomes the sequence bias observed when digesting chromatin with restriction enzymes giving you better ultra-resolution and more chromatin centric answers.
The Micro-C kit sees what you’ve been missing, unveiling the approximately 20% of the mappable human genome hidden to restriction enzyme-based Hi-C methods. Unlock the 3D genome architecture at the nucleosome level and start discovering the epigenetic answers you’ve been looking for.
3D Data at a Fraction of the Sequencing Cost
The ability to detect higher-order features, such as chromatin loops, in proximity ligation data is dependent on enriching long-range informative reads to capture chromatin interaction frequency. The Dovetail Micro-C Kit only requires 800 M reads to get 1 kb matrices compared to the 1.2-1.6 billion reads required by restriction enzyme-based Hi-C methods. Translation? Significant sequencing savings for you.
Hit the Ground Running with the Micro-C Kit
Dovetail Genomics® has done all the reagent qualification and protocol optimization for you, eliminating the finicky sonication step used by other methods to improve reproducibility. The Micro-C kit is simple to use so you can get answers sooner. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.
Struggling to link a phenotype with a mechanism of action? The Micro-C kit can potentially help with that. Wondering what is occurring within a TAD? The Micro-C kit can tell you. Curious how topology influences gene regulation and controls enhancer/promoter interactions? Find out with the Micro-C kit!
Technology | Micro-C Video
Going from Sample to NGS Library
Catalog # 25004
Catalog # 25010
|Validated Samples||Mammalian cells|
|Labeling||Research Use Only|
|Application(s)||Chromatin conformation analysis|
|Module 1 of 2 – storage||2°C to 8°C|
|Module 1 of 2 – content||TE Buffer, pH 8.0
10x Wash Buffer
2x NTB Solution
Chromatin Capture Beads
Crosslink Reversal Buffer
10x RBC Lysis Buffer
|Module 2 of 2 – storage||-30°C to -10°C|
|Module 2 of 2 – content||MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
5x Bridge Ligation Buffer
5x Bridge Ligation (T4 DNA Ligase)
250 mM DTT
HotStart PCR Ready Mix