Dovetail® Micro-C Kit

Catalog # 21006

Dovetail® Micro-C Kit

Catalog # 21006

Push Hi-C resolution limits with the Dovetail® Micro-C Kit. The Micro-C kit uses micrococcal nuclease (MNase) to digest chromatin prior to proximity ligation to generate ultra-high-resolution 3D genome architecture mapping. Only need high-resolution data? Save on sequencing costs compared to restriction enzyme-based methods when building 1 kb matrices.

The kit provides all the optimized reagents necessary for performing the proximity ligation steps on mammalian samples prior to NGS library generation. Add this to other ready-to-use Dovetail® kits and you’ll have a sequence ready library in no time.

The MNase Advantage

The MNase enzyme possesses both sequence-independent endonuclease and exonuclease activities to generated uniform fragments that are nucleosome length (146 bp). This overcomes the sequence bias observed when digesting chromatin with restriction enzymes giving you better ultra-resolution and more chromatin centric answers.

The Micro-C kit sees what you’ve been missing, unveiling the approximately 20% of the mappable human genome hidden to restriction enzyme-based Hi-C methods. Unlock the 3D genome architecture at the nucleosome level and start discovering the epigenetic answers you’ve been looking for.

3D Data at a Fraction of the Sequencing Cost

The ability to detect higher-order features, such as chromatin loops, in proximity ligation data is dependent on enriching long-range informative reads to capture chromatin interaction frequency. The Dovetail Micro-C Kit only requires 800 M reads to get 1 kb matrices compared to the 1.2-1.6 billion reads required by restriction enzyme-based Hi-C methods. Translation? Significant sequencing savings for you.

Hit the Ground Running with the Micro-C Kit

Dovetail Genomics® has done all the reagent qualification and protocol optimization for you, eliminating the finicky sonication step used by other methods to improve reproducibility. The Micro-C kit is simple to use so you can get answers sooner. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Struggling to link a phenotype with a mechanism of action? The Micro-C kit can potentially help with that. Wondering what is occurring within a TAD? The Micro-C kit can tell you. Curious how topology influences gene regulation and controls enhancer/promoter interactions? Find out with the Micro-C kit!

Technology | Micro-C Video

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Going from Sample to NGS Library

Dovetail® Micro-C Kit

Catalog # 21006

Pan Promoter Enrichment Kit

Catalog # 25013 (Human)
Catalog # 25014 (Mouse)

Catalog # 25004

Catalog # 25010

Specifications

Reactions 8
Validated Samples Mammalian cells
Technique Hi-C
Labeling Research Use Only
Application(s) Chromatin conformation analysis
Module 1 of 2 – storage 2°C to 8°C
Module 1 of 2 – content TE Buffer, pH 8.0
10x Wash Buffer
TWB Solution
2x NTB Solution
LWB Solution
NWB Solution
Chromatin Capture Beads
Crosslink Reversal Buffer
Streptavidin Beads
10x RBC Lysis Buffer
20% SDS
Module 2 of 2 – storage -30°C to -10°C
Module 2 of 2 – content MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
Bridge
5x Bridge Ligation Buffer
5x Bridge Ligation (T4 DNA Ligase)
Proteinase K
250 mM DTT
HotStart PCR Ready Mix

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