Epigenetics – HiChIP MNase Service

Catalog # 20017

Epigenetics – HiChIP MNase Service

Catalog # 20017

Dovetail® HiChIP combines ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Choose from one of our three antibodies – CTCF, H3K4me3, and H3K27 – to map protein-directed chromatin conformation in your human or mouse derived cell or tissue samples to elucidate novel epigenetic mechanisms. You provide the sample(s), we’ll get you the data.

Just want us to generate the NGS library so you can perform your own sequencing and analysis? No problem. Want Dovetail to take on your HiChIP project, from sample to sequence data (we deliver a minimum of 200M read-pairs per sample and contact matrices ready for downstream analysis and interpretation)? Just let us know.

Why Use Our  Service

• Discover enhancer-promoter interactions controlling expression of your gene of interest – Uncover not just where proteins bind, but also the long-range interactions they mediate

• Get ultra-high nucleosome-level resolution of chromatin contacts – MNase generates nucleosome length (146 bp) fragments and proximity ligation is optimized to maximize long-range interaction

• With protein-directed enrichment, boost your signal-to-noise and enrichment of long-range informative reads

• Add meaning to your ChIP-seq data by uncovering the interactome associated with your protein of interest

How It Works

Your designated Scientific Project Manager will work with you to understand your goals and define deliverables specific to your project. We can complete your project from beginning to end or just perform part of it – it’s totally up to you!

Just prep your samples using our best practices for transfer using the best practices guidelines we’ve established, send us your mammalian cells, tissue, or blood samples, and sit back while our Hi-C experts do the work for you! Once we receive your sample(s), your Scientific Project Manager will keep you informed of our progress as we hit each project milestone.

Learn more about the HiChIP MNase Kit

Specifications

Delivery Time 10 weeks from sample to completed analysis
Sample Type Human or mouse cells, tissue, or blood
Library Generation Dovetail Hi-C
Sequencing Platform Illumina
Analysis Platform Dovetail QC pipeline
Available Antibodies CTCF, H3K4me3, or H3K27ac (IgG available as a negative control)
Available Add-On ChIP-seq MNase Service
Potential Deliverables QC Report
Sequence data (*.fastq file format)
Alignment (.bam format)
Contact matrices (*.mcool and *.hic file formats)
Pairs file (.pair format)

Resources

Webinars

Product Literature

Scientific Literature