• Fully leverage our complete sample-to-publishable assembly workflow.
• Trust our qualified annotation bioinformaticians to deliver on this crucial final step in genome assembly.
• High-throughput, bespoke annotation pipeline to maximize speed and performance.

Maximize your genome assembly investment with accurate and complete gene annotation. Gene annotation errors will derail your downstream analyses. The experts at Dovetail can provide you with an annotated assembly you can trust. To confidently call genes, we require evidence from as many trustworthy sources as possible, which may include publicly available transcriptome data, protein databases, etc. However, the best evidence is RNAseq or IsoSeq data from a variety of tissues and/or life stages from the species under study. If transcriptome data is unavailable, we can generate it for you as part of our complete service offering. Genes will be called, identified, and functionally annotated. Then a qualified bioinformatician will manually curate up to 5 genes (Basic package) or 55 genes (Extended package) of your choice.

The annotation workflow includes:

• Repeat masking
• Model preparation
• Evidence collection
• High throughput genome annotation
• Manual curation
• Functional annotation

Following ab initio annotation, a trained bioinformatician will manually curate genes most relevant to your research. In this example, IsoSeq data (A) clearly shows the presence of an exon that the ab initio pipeline missed. Similarly, IsoSeq data (B) does not support an exon that the ab initio pipeline erroneously called in the genome. Manual curation ensures optimal annotation accuracy.