Dovetail® Scaffolding
Catalog # 20011

Why Add a Scaffolding Project to your De Novo Genome Assembly Service?
For the vast majority of genomes, long-read sequencing is insufficient to yield a chromosome-scale assembly. A Dovetail® Scaffolding Project will improve contiguity for the highest quality assembly and will enable you to:
- Enjoy full service – from sample to publishable assembly under one roof.
- Get access to the latest cutting-edge technologies: PacBio HiFi, Dovetail Omni-C, HiRise software and the Dovetail® Annotation Service Suite.
- Receive a full genome: don’t settle for half a genome – new chromosomal-scale phased SNP calling for 2021 assemblies!
How It Works
Ship us a flash-frozen tissue sample and upload your existing assembly with scaffold N50 >100kb. A qualified bioinformatician will check the assembly for accuracy and completeness. If your assembly passes our QC thresholds, a dedicated project manager will set up your scaffolding project.
We will construct Omni-C proximity ligation libraries, QC the libraries, deep sequence with Illumina PE 150, and scaffolding using the HiRise® algorithm. Topologically associated domains (TADs) will be reported if present. A detailed project report will accompany your high quality, chromosome-scale assembly. Post-project discussion with your project manager is available upon request.


A. ipaensis & A. duranensis
Dovetail Hi-C produced incredible scaffold ordering for our assemblies of the diploid ancestral species of peanut, A. duranensis and A. ipaensis. The new orderings made even clearer the incredibly close relationships between the genomes of these diploid species and the tetraploid crop.
– David Bertioli, University of Georgia

Specifications
Delivery Time | Inquire |
Sample Requirement | An input draft assembly with >100 kb N50 and most contigs over 1Kb, a tissue or DNA sample of sufficient quality, and any species-specific information (e.g. predicted genome size). |
Library | Dovetail Omni-C Library |
Sequencing Platform | Illumina |
Analysis Platform | HiRise (scaffolding) |
Potential Project Deliverables | Final Dovetail Genome Assembly
Chromatin Topology TAD analysis
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