Why Use Our Assembly Scaffolding Service?
For the vast majority of genomes, long-read sequencing is insufficient to yield a chromosome-scale assembly. Increase the contiguity of your assembly up to chromosome-scale with Omni-C restriction enzyme-free proximity ligation and HiRise software. You’ll get:
- Full service from sample to scaffolded assembly.
- Unbiased DNaseI-based chromatin digestion for even whole genome coverage.
- Manual correction for optimal accuracy.
- Dedicated project manager for pre- and post-project support.
How It Works
Ship us a flash-frozen tissue sample and upload your existing assembly with scaffold N50 >100kb. A qualified bioinformatician will check the assembly for accuracy and completeness. If your assembly passes our QC thresholds, a dedicated project manager will set up your scaffolding project.
We will construct Omni-C proximity ligation libraries, QC the libraries, deep sequence with Illumina PE 150, and scaffold using HiRise®. Topologically associated domains (TADs) will be reported if present. A detailed project report will accompany your high quality, chromosome-scale assembly. Post-project discussion with your project manager is available upon request.
For our conservation genomics project, we provided Dovetail with a precious and limited sample from our species of interest. We were blown away by the completeness and of the chromosome-scale assembly they produced using HiFi, Omni-C, and the HiRise pipeline. With an assembly drastically exceeding Vertebrate Genomes Project standards in hand, we can put all our focus on the downstream science.
– Charles Feigin, Princeton University
|Sample Requirements||An input draft assembly with >100 kb scaffold N50 and most contigs over 1Kb, a tissue or DNA sample of sufficient quality, and any species-specific information (e.g. estimated genome size).|
|Library||Dovetail Omni-C Library|
|Analysis Platform||HiRise (scaffolding)|
|Project Deliverables||HiRise Assembly deliverables:
TAD Analysis deliverables, if applicable, include: