Dovetail Genomics is the leader in the field of chromatin conformation capture (HiC). Watch this video to learn more about our novel solutions enabling new science in the fields of epigenetics, developmental biology, drug discovery, cancer research, and evolutionary biology.
The Dovetail® HiChIP MNase combines the benefits of ChIP-seq wiht the long-range information of Hi-C, enabling genome wide mapping of protein-directed topological features. Identify locally bound-sequences and distal interactions up to megabases away, mediate by your protein of interest (e.g. promoter/enhahcer interactions). Learn more!
The Dovetail® Micro-C Kit enables capture of chromatin topology at a mono-nucleosome resolution. Watch this webinar and discover how you can get the highest resolution achievable with uniform sequence coverage or save on sequencing costs. Enjoy the next evolution to Hi-C for high-resolution mapping of chromatin topological features.
In this webinar, learn how:
– Using MNase delivers the highest resolution in chromatin conformation
– Increased signal-to-noise ratios translates to reduced sequencing burdens
– Micro-C captures features that other Hi-C approaches have difficultly detecting
– Micro-C an be leveraged in protein-direct approaches to chromatin capture assays
Define Chromatin Architecture at the Nucleosome Level
From the creators of the Dovetail® HiChIP and Micro-C assays respetively, Marco Blanchette (VP R&D) and LIsa Munding (Lead Scientist) share the history behind developing these game changing assay kits for epigenetic research. Hear how the kits work, their benefits, and the ways Marco and Lisa see these new technologies impacting genomics research.
During this seminar, you’ll learn how:
– Hi-C addresses a variety of epigenetics questions
– The Dovetail® Omni-C® Kit expands the utility of Hi-C to include WGS applications
– The Dovetail® Micro-C Kit generates high resolution views of genome topology
– The Dovetail® HiChIP MNase Kit maps protein-directed chromatin architecture
What you’ll learn:
– What is loop calling when it comes to HiChIP
– The tool landscape: the trade-off’s and benefits
– How to identify statistically significant interactions
– How to plot HiChIP arcs in R
– Calling 1-D peaks with MACS2 with HiChIP data
In this talk, Dr. Hockemeyer will discuss the use of Dovetail® Hi-C technology to assemble Indian and Chinese muntjac genomes to investigate telomere biology and chromosome fusion events in human cancer.
Linking Sequence Variation to Chromatin Dynamics through Genome Topology
Cory Padilla, Ph.D.
Key discussion points:
– The role of genome conformation in gene regulation
– Why integrating conformation data with genotyping data can provide a more complete view of variants
– How to capture variants (SNVs and structural variants) with conformation in a single sequencing library
Directed Evolution of Genome Assemblies - and Why it is Important
Harris Lewin, Ph.D.
Trace the directed evolution of 28 genome assemblies of the Narwhal, an iconic cetacean known at the “unicorn of the sea”. While understanding the evolution and amazing phenotypes of the Narwhal is the ultimate goal, the evolution of genome assemblies from short-read to long read-based assemblies with Dovetail scaffolding has been an interesting scientific journey in itself. Different sequencing approaches will be discussed in the light of genome assembly evolution, and their importance for understanding genome biology.
Assembling a Reference-Quality Genome for Solemya velum (Bivalvia: Protobranchia)
Vanessa L. González, Ph.D.
We generated a reference-quality genome Solemya velum (Bivalvia: Protobranchia) to allow us to test hypotheses about mollusc genome evolution and the symbiotic interactions in marine molluscs. 10X Genomics, PacBio Long-reads and Hi-C data was leveraged for molluscan genome assembly. Iterations and combinations of each data type have resulted in a 2.4 GB reference genome with a scaffold N50 of 200 MB and a BUSCO completeness score of 93.7%.
Sequencing & Assembling the Mega-Genomes of Mega-Trees
Steven Salzberg, Ph.D.
Johns Hopkins University
The giant sequoia and the coast redwood are two of the largest living organisms. We’ll discuss our successful genome assembly of a 1360-year-old sequoia that produced the largest scaffold of any genome ever attempted and our near-complete work on the genome of a 1390-year-old coast redwood. Both projects used a combination of short reads and long Oxford Nanopore reads for initial assembly, and Hi-C linked reads from Dovetail Genomics for scaffolding.