Dovetail® Targeted Enrichment Panels
Catalog # 25013 (Human)
Catalog # 25014 (Mouse)
What is Targeted Enrichment?
Next-generation sequencing (NGS) is an extremely powerful tool for capture genome-wide genetic information. However, more targeted investigations can be performed by hybridizing the sequencing library to capture probes that target pre-selected genomic regions and “pulling down” the captured duplexes prior to sequencing, thereby enriching your library. The result is a reduction in sequencing required to meeting the sequence coverage goal for your targeted regions.
Target Enrichment Applied to Proximity Ligation
Targeted enrichment can be combined with proximity ligation libraries to perform capture Hi-C. However, library bias due to unevenly distributed restriction sites must be considered during the enrichment panel design process since they can cause coverage bias in the final library. Dovetail® Micro-C and Omni-C® libraries use sequence-independent nucleases to eliminate this source of bias so standard design principles can be employed. While many commercially available targeted enrichment panels are compatible with our kits, only the Dovetail Pan Promoter Enrichment Panels have been designed specifically for the study of chromatin topology anchored at gene promoter sites.
Available Enrichment Panels
Dovetail® Pan Promoter Enrichment Panels target over 84,000 and 47,000 human and mouse promoter sequences respectively, offering comprehensive coverage of the promoter landscape. This inclusive design offers a genome-wide view of interactions anchored at known promoter sites enabling the detection of promoter-specific interactions such as promoter-enhancer and promoter-promoter contacts.
Comprehensive Coverage of Promoter Regions
Covering greater than 98% of human and mouse promoter regions, Dovetail Pan Promoter Enrichment panels are perfect for probing known and discovering unknown distal regulatory elements interacting with known promoters. The human design covers over 80,000 promoters associated with more than 27,000 coding and non-coding genes, while the mouse design covers over 47,000 promoters and 24,000 coding and non-coding genes.
Sequencing Cost-Saving Compared to Standard Hi-C
Standard Hi-C methods enable detection of promoter interactions through the calling of chromatin loops at high resolution (typically 5kb or greater). To achieve sufficient read depth to support these calls, sequencing depths in excess of 1 billion reads are required. Dovetail Pan Promoter Enrichment Panels reduce sequencing 10-fold without compromising coverage over promoter regions.
Data Confidence with High Enrichment Reproducibility
Within each panel, enrichment probes are balanced, and enrichment conditions optimized for consistent experiment to experiment results.
See More: Higher Resolution View of Promoter Specific Interactions
Targeted approaches reduce overall library complexity by improving signal-to-noise, both increasing representation of contacts of interest and reducing overall background signal. The result is a more efficient use of sequence and greater evidence of contacts within regions of interest.
Easily Integrate Capture Hi-C Into Your Existing Workflow
Hybridization capture is performed on the final proximity ligation libraries immediately prior to sequencing, so you won’t need to make any change to the upfront proximity ligation workflow. Existing Dovetail Omni-C and Micro-C libraries can be target enriched without the need to create new libraries for this purpose.
|Reaction||4 with up to 8 libs multiplexed/rxn|
|Maximum # Libraries||32|
|Validated Samples||Human or Mouse/span|
|Compatible Kits||Dovetail Omni-C & Micro-C Kits|
|Labeling||For Research Use Only|
|Module 1 of 3 – content||Streptavidin Binding Beads
DNA Purification Beads
Wash Buffer 1
Wash Buffer 2
|Module 1 of 3 – storage||2˚C to 8˚C|
|Module 2 of 3 – storage||-25˚C to -15˚C|
|Module 2 of 3 – content||Universal Blockers
Capture Amplification Mix
|Module 3 of 3 – storage||-25˚C to -15˚C|
|Module 3 of 3 – content||Pan Promoter Panel|