Dovetail® Omni-C® Kit

Catalog # 21005

Dovetail® Omni-C® Kit

Catalog # 21005

The Dovetail® Omni-C® Kit has been optimized to deliver uniform Hi-C data with per-base-coverage approximating standard shot-gun libraries that still delivers the highest degree of long-range information. Extract more data from every sequencing run to comprehensively understand genetic variants like SNPs and large SVs in a single assay.

The Omni-C Kit provides all the reagents necessary for performing the proximity ligation steps on plant and animal samples prior to NGS library generation. Add this to other ready-to-use Dovetail® kits and you’ll have a sequence ready library in no time.

Genome-Wide Resolution of Chromatin Interactions

The Omni-C kit has optimized DNase I, a sequence-independent endonuclease, to reproducibly digest chromatin, delivering all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme-based Hi-C approaches.

Get enriched long-range cis reads, and more complete contact matrices when viewing chromatin conformation and looping interactions. Omni-C data gives you the most complete genome-wide view for all your genome assembly, phasing, structural variant, and SNP research.

Uncover the role of SNPs, SVs, and Chromosome Phasing

Unsure how single nucleotide polymorphisms (SNPs), structural variants (SVs), or chromosome phasing impacts disease progression? Want to know if a SNP impacts a gene a megabase away? The Omni-C kit lets you dive deeper into the mechanisms of action to help you find answers. The improved coverage of Omni-C libraries enables genome-wide SNP calling, generate high-resolution data that can detect large structural variants, and deliver chromosome phasing information with low switch error rates – all with one library. Get a better understanding of the relationship between the genome and phenotype to potentially uncover the missing link for a particular disease.  Elucidate mechanisms associated with SNPs that fall into non-coding regions to annotate their functional significance.

Optimized Protocols Delivered Straight to Your Hands

Dovetail Genomics® has done all the protocol optimization and reagent qualification for you, so you can hit the ground running. The protocol eliminates the finicky sonication step used by other methods to improve reproducibility and is validated and well documented to ensure the data quality. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Technology | Omni-C Video

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Going from Sample to NGS Library

Catalog # 21005

Dovetail® Library Module for Illumina

Catalog # 25004

Dovetail® Dual Index Primer Set #1 for Illumina

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Specifications

Reactions 8
Validated Samples Mammalian cells
Technique Hi-C, ChIP-seq
Labeling Research Use Only
Applications Protein directed chromatin conformation, gene-regulation & discovery of novel enhancers and super-enhancers
Module 1 of 2 – storage 2°C to 8°C
Module 1 of 2 – content TE Buffer, pH 8.0
10x Wash Buffer
TWB Solution
2x NTB Solution
NWB Solution
Protein A/G Beads
10x Crosslink Reversal Buffer
Streptavidin Beads
10x RBC Lysis Buffer
20% SDS
Module 2 of 2 – storage -30°C to -10°C
Module 2 of 2 – content MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl<2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
Bridge
5x Bridge Ligation Buffer
T4 DNA Ligase
Proteinase K
250 mM DTT
HotStart PCR Ready Mix

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