Dovetail® Omni-C® Kit

Catalog # 21005

Dovetail® Omni-C® Kit

Catalog # 21005

The Dovetail® Omni-C® Kit has been optimized to deliver uniform Hi-C data with per-base-coverage approximating standard shot-gun libraries that still delivers the highest degree of long-range information. Extract more data from every sequencing run to comprehensively understand genetic variants like SNPs and large SVs in a single assay.

The Omni-C Kit provides all the reagents necessary for performing the proximity ligation steps on plant and animal samples prior to NGS library generation. Add this to other ready-to-use Dovetail® kits and you’ll have a sequence ready library in no time.

Genome-Wide Resolution of Chromatin Interactions

The Omni-C kit has optimized DNase I, a sequence-independent endonuclease, to reproducibly digest chromatin, delivering all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme-based Hi-C approaches.

Get enriched long-range cis reads, and more complete contact matrices when viewing chromatin conformation and looping interactions. Omni-C data gives you the most complete genome-wide view for all your genome assembly, phasing, structural variant, and SNP research.

Uncover the role of SNPs, SVs, and Chromosome Phasing

Unsure how single nucleotide polymorphisms (SNPs), structural variants (SVs), or chromosome phasing impacts disease progression? Want to know if a SNP impacts a gene a megabase away? The Omni-C kit lets you dive deeper into the mechanisms of action to help you find answers. The improved coverage of Omni-C libraries enables genome-wide SNP calling, generate high-resolution data that can detect large structural variants, and deliver chromosome phasing information with low switch error rates – all with one library. Get a better understanding of the relationship between the genome and phenotype to potentially uncover the missing link for a particular disease.  Elucidate mechanisms associated with SNPs that fall into non-coding regions to annotate their functional significance.

Optimized Protocols Delivered Straight to Your Hands

Dovetail Genomics® has done all the protocol optimization and reagent qualification for you, so you can hit the ground running. The protocol eliminates the finicky sonication step used by other methods to improve reproducibility and is validated and well documented to ensure the data quality. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Technology | Omni-C Video

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Going from Sample to NGS Library

Dovetail® Library Module for Illumina

Catalog # 25004

Dovetail® Dual Index Primer Set #1 for Illumina

Catalog # 25010


Reactions 8
Validated Samples Mammalian cells, mammalian and non-mammalian animal tissues, cyropreserved PBMCs, fresh mammalian whole blood, nucleated blood, and plants
Technique Hi-C
Labeling Research Use Only
Applications Genome Assembly, Chromatin conformation analysis, SNP and structural variant detection
Module 1 of 2 – storage 2°C to 8°C
Module 1 of 2 – content 10x Wash Buffer
NWB Solution
Chromatin Capture Beads
Streptavidin Beads
20% SDS
Crosslink Reversal buffer
Module 2 of 2 – storage -30°C to -10°C
Module 2 of 2 – content

Nuclease Enzyme Mix
10X Nuclease Digest Buffer
100 mM MnCI2
End Polishing Enzyme Mix
End Polishing Buffer
5X Bridge Ligation Buffer
Bridge Ligase
Intra-Aggregate Ligation Enzyme Mix
Intra-Aggregate Ligation Buffer
Proteinase K
250 mM DTT
HotStart PCR Ready Mix


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