Dovetail® Omni-C® Kit
Catalog # 21005
Dovetail® Omni-C® Kit
Catalog # 21005
Genome-Wide Resolution of Chromatin Interactions
The Omni-C kit has optimized DNase I, a sequence-independent endonuclease, to reproducibly digest chromatin, delivering all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme-based Hi-C approaches.
Get enriched long-range cis reads, and more complete contact matrices when viewing chromatin conformation and looping interactions. Omni-C data gives you the most complete genome-wide view for all your genome assembly, phasing, structural variant, and SNP research.
Uncover the role of SNPs, SVs, and Chromosome Phasing
Unsure how single nucleotide polymorphisms (SNPs), structural variants (SVs), or chromosome phasing impacts disease progression? Want to know if a SNP impacts a gene a megabase away? The Omni-C kit lets you dive deeper into the mechanisms of action to help you find answers. The improved coverage of Omni-C libraries enables genome-wide SNP calling, generate high-resolution data that can detect large structural variants, and deliver chromosome phasing information with low switch error rates – all with one library. Get a better understanding of the relationship between the genome and phenotype to potentially uncover the missing link for a particular disease. Elucidate mechanisms associated with SNPs that fall into non-coding regions to annotate their functional significance.
Optimized Protocols Delivered Straight to Your Hands
Dovetail Genomics® has done all the protocol optimization and reagent qualification for you, so you can hit the ground running. The protocol eliminates the finicky sonication step used by other methods to improve reproducibility and is validated and well documented to ensure the data quality. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.
Technology | Omni-C Video
Specifications
| Reactions | 8 |
| Validated Samples | Plant and animal cells and tissues |
| Technique | Hi-C |
| Labeling | Research Use Only |
| Applications | Genome Assembly, Chromatin conformation analysis, SNP and structural variant detection |
| Module 1 of 2 – storage | 2°C to 8°C |
| Module 1 of 2 – content | TE Buffer, pH 8.0 10x Wash Buffer TWB Solution 2x NTB Solution LWB Solution NWB Solution Chromatin Capture Beads 10x Crosslink Reversal Buffer Streptavidin Beads 10x RBC Lysis Buffer 20% SDS 10% Triton X-100 100 mM MnCl2 0.5 M EDTA 20% SDS |
| Module 2 of 2 – storage | -30°C to -10°C |
| Module 2 of 2 – content | Nuclease Enzyme Mix 10x Nuclease Digest Buffer End Polishing Enzyme Buffer End Polishing Enzyme Mix Intra-Aggregate Ligation Buffer Intra-Aggregate Ligation Enzyme Mix T4 DNA Ligase Bridge 5x Bridge Ligation Buffer Proteinase K 250 mM DTT HotStart PCR Ready Mix |