Dovetail® HiChIP MNase Kit
Catalog # 21007
Dovetail® HiChIP MNase Kit
Catalog # 21007


See Your ChIP-seq Data in a Whole New Dimension
Genomic interactions matter. With ChIP-seq, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.
HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.


Get Nucleosome-Level Resolution…
Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).




…or 3D data at a fraction of the cost
Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.


Simple, Reproducible Workflow in an Easy-to-Use Kit
Go from sample to a sequence in just 2.5 days. Dovetail Genomics® kits are ready-to-use out of the box so you don’t need to spend time optimizing your protocol or qualifying reagents. A novel dual-crosslinking approach delivers unmatched capture of long-range interactions and library complexity so you can analyze chromatin interactions from nucleosomes to megabases. Plus, using MNase eliminates the need to sonicate your sample, removing one of the biggest sources of experimental variability. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.




Visayan Warty Pig


We are very pleased with the result of the assembly. It really is beyond our expectations. The first analyses have already shown some very nice rearrangements compared to the genomes of other closely related species. I also am very positive how you handled the whole process and kept us informed about the status during the different steps of the assembly.
– Martien Groenen, Wageningen University


Technology | HiChIP Video
Validated Antibodies
Dovetail Genomics has validated the following commercial antibodies so you can hit the ground running.
Dovetail® HiChIP Validated Antibody |
Supplier | Part Number |
---|---|---|
IgG | Cell Signaling | 2729 |
CTCF | Cell Signaling | 3418 |
H3K4ac | Active Motif | 39381 |
H3K4me3 | Cell Signaling | 9751 |
H3K14ac | Cell Signaling | 7627 |
H3K27 | Cell Signaling | 8173 |
H3K27me3 | Cell Signaling | 9733 |
Dovetail® HiChIP Validated Antibody |
Supplier | Part Number |
---|---|---|
H3K36me3 | Cell Signaling | 4909 |
Oct4 | Cell Signaling | 2890 |
Sox2 | Cell Signaling | 23064 |
Klf4 | Abcam | ab106629 |
Nanog | Abcam | ab21624 |
PolII | Abcam | ab26721 |
Specifications
Reactions | 8 |
Validated Samples | Mammalian cells |
Technique | Hi-C, ChIP-seq |
Labeling | Research Use Only |
Applications | Protein directed chromatin conformation, gene-regulation & discovery of novel enhancers and super-enhancers |
Module 1 of 2 – storage | 2°C to 8°C |
Module 1 of 2 – content | TE Buffer, pH 8.0 10x Wash Buffer TWB Solution 2x NTB Solution NWB Solution Protein A/G Beads 10x Crosslink Reversal Buffer Streptavidin Beads 10x RBC Lysis Buffer 20% SDS |
Module 2 of 2 – storage | -30°C to -10°C |
Module 2 of 2 – content | MNase Enzyme Mix 10x Nuclease Digest Buffer 100 mM MgCl2 0.5 M EGTA End Polishing Enzyme Buffer End Polishing Enzyme Mix Intra-Aggregate Ligation Buffer Intra-Aggregate Ligation Enzyme Mix Bridge 5x Bridge Ligation Buffer T4 DNA Ligase Proteinase K 250 mM DTT HotStart PCR Ready Mix |