Dovetail^® HiChIP MNase Kit

Catalog # 21007

Dovetail^® HiChIP MNase Kit

Catalog # 21007

The Dovetail^® HiChIP MNase Kit combines the benefits of ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Query protein-directed chromatin conformation mediated by specific proteins of interest and see your ChIP-seq data in a whole new dimension.

The kit provides optimized reagents needed to perform the ChIP and proximity ligation steps prior to NGS library generation and is compatible with pre-validated commercially available antibodies. Add this to other ready-to-use Dovetail kits and you’ll have a sequence ready library in no time.

See Your ChIP-seq Data in a Whole New Dimension

Genomic interactions matter. With ChIP-seq, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.

HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.

Get Nucleosome-Level Resolution…

Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).

…or 3D data at a fraction of the cost

Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.

Simple, Reproducible Workflow in an Easy-to-Use Kit

Go from sample to a sequence in just 2.5 days. Dovetail Genomics® kits are ready-to-use out of the box so you don’t need to spend time optimizing your protocol or qualifying reagents. A novel dual-crosslinking approach delivers unmatched capture of long-range interactions and library complexity so you can analyze chromatin interactions from nucleosomes to megabases. Plus, using MNase eliminates the need to sonicate your sample, removing one of the biggest sources of experimental variability. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Visayan Warty Pig

We are very pleased with the result of the assembly. It really is beyond our expectations. The first analyses have already shown some very nice rearrangements compared to the genomes of other closely related species. I also am very positive how you handled the whole process and kept us informed about the status during the different steps of the assembly.

– Martien Groenen, Wageningen University

Technology | HiChIP Video

3:53

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Validated Antibodies

Dovetail Genomics has validated the following commercial antibodies so you can hit the ground running.

Dovetail^® HiChIP Validated Antibody	Supplier	Part Number
IgG	Cell Signaling	2729
CTCF	Cell Signaling	3418
H3K4ac	Active Motif	39381
H3K4me3	Cell Signaling	9751
H3K14ac	Cell Signaling	7627
H3K27	Cell Signaling	8173
H3K27me3	Cell Signaling	9733

Dovetail^® HiChIP Validated Antibody	Supplier	Part Number
H3K36me3	Cell Signaling	4909
Oct4	Cell Signaling	2890
Sox2	Cell Signaling	23064
Klf4	Abcam	ab106629
Nanog	Abcam	ab21624
PolII	Abcam	ab26721

Going from Sample to NGS Library