Dovetail® HiChIP MNase Kit

Catalog # 21007

Dovetail® HiChIP MNase Kit

Catalog # 21007

The Dovetail® HiChIP MNase Kit combines the benefits of ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Query protein-directed chromatin conformation mediated by specific proteins of interest and see your ChIP-seq data in a whole new dimension.

The kit provides optimized reagents needed to perform the ChIP and proximity ligation steps prior to NGS library generation and is compatible with pre-validated commercially available antibodies. Add this to other ready-to-use Dovetail kits and you’ll have a sequence ready library in no time.

See Your ChIP-seq Data in a Whole New Dimension

Genomic interactions matter. With ChIP-seq, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.

HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.

CTCF ChIP-seq versus Dovetail HiChIP

Get Nucleosome-Level Resolution…

Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).

…or 3D data at a fraction of the cost

Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.

Simple, Reproducible Workflow in an Easy-to-Use Kit

Go from sample to a sequence in just 2.5 days. Dovetail Genomics® kits are ready-to-use out of the box so you don’t need to spend time optimizing your protocol or qualifying reagents. A novel dual-crosslinking approach delivers unmatched capture of long-range interactions and library complexity so you can analyze chromatin interactions from nucleosomes to megabases. Plus, using MNase eliminates the need to sonicate your sample, removing one of the biggest sources of experimental variability. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Visayan Warty Pig

We are very pleased with the result of the assembly. It really is beyond our expectations. The first analyses have already shown some very nice rearrangements compared to the genomes of other closely related species. I also am very positive how you handled the whole process and kept us informed about the status during the different steps of the assembly.

Martien Groenen, Wageningen University

Technology | HiChIP Video

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Validated Antibodies

Dovetail Genomics has validated the following commercial antibodies so you can hit the ground running.

Dovetail® HiChIP
Validated Antibody
Supplier Part Number
IgG Cell Signaling 2729
CTCF Cell Signaling 3418
H3K4ac Active Motif 39381
H3K4me3 Cell Signaling 9751
H3K14ac Cell Signaling 7627
CH3K27ac Cell Signaling 8173
H3K27me3 Cell Signaling 9733
Dovetail® HiChIP
Validated Antibody
Supplier Part Number
H3K36me3 Cell Signaling 4909
SMC3 Cell Signaling ab9263
Oct4 Cell Signaling 2890
Sox2 Cell Signaling 23064
Klf4 Cell Signaling ab106629
Nanog Cell Signaling ab21624
PolII Cell Signaling 61667

Going from Sample to NGS Library

Dovetail® HiChIP MNase Kit

Catalog # 21007

Dovetail® Library Module for Illumina

Catalog # 25004

Dovetail® Dual Index Primer Set #1 for Illumina

Catalog # 25010

Specifications

Reactions 8
Validated Samples Mammalian cells
Technique Hi-C, ChIP-seq
Labeling Research Use Only
Applications Protein directed chromatin conformation, gene-regulation & discovery of novel enhancers and super-enhancers
Module 1 of 2 – storage 2°C to 8°C
Module 1 of 2 – content TE Buffer, pH 8.0
10x Wash Buffer
TWB Solution
2x NTB Solution
NWB Solution
Protein A/G Beads
10x Crosslink Reversal Buffer
Streptavidin Beads
10x RBC Lysis Buffer
20% SDS
Module 2 of 2 – storage -30°C to -10°C
Module 2 of 2 – content MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
Bridge
5x Bridge Ligation Buffer
T4 DNA Ligase
Proteinase K
250 mM DTT
HotStart PCR Ready Mix

Resources

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Data Processing

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