Omni-C™ Kit

Catalogue #: 21005

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Specifications

Reactions

8

Validated Samples

Mammalian cells and tissues

Technique

Hi-C

Labeling

Research Use Only

Application(s)

Chromatin conformation analysis, SNP and structural variant detection

Product Description

The Dovetail™ Omni-C™ Kit uses a sequence independent endonuclease for chromatin digestion prior to proximity ligation. The Omni-C™ Kit provides all the reagents necessary for performing the proximity ligation steps on mammalian samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard pair-end library preparation kits for Illumina sequencing.

The Omni-C Kit offers the following key benefits:

  • Sequence-independent chromatin fragmentation enables genome-wide detection of chromatin contacts (up to 20% of the genome lacks coverage using restriction enzyme based Hi-C approaches)
  • Even genome-wide coverage, similar to shotgun sequencing, enables SNP calling, chromosome phasing, and structural variant detection
  • Lower sequencing burden to reach desired depth of coverage saves time and cost

Omni-C Libraries Enable Genome Wide Resolution of Chromatin Interactions

Due to the use of a sequence independent endonuclease for chromatin digestion, the Omni-C Kit offers all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme based Hi-C approaches. Compared to restriction enzyme-based Hi-C approaches, Omni-C provides the following benefits:

  • Significant overlap with data generated using restriction enzyme based approaches but enriched in long-range cis reads
  • Improved resolution when viewing chromatin conformation and looping interactions
  • Most complete view of genome-wide chromatin conformation through dramatically increased resolution of topological interactions that occur in regions with low restriction enzyme density

SNPs & Chromosome Phasing

The even sequence coverage associated with Omni-C libraries enables genome-wide SNP calling and downstream applications based on SNP information. For example:

  • Improved coverage of Omni-C libraries enables chromosome phasing with low switch error rates
  • Best possible approach for whole-genome physical phasing using Illumina short reads

Large SVs Are Captured In Omni-C Data

The proximity ligation data can be used to detect and confirm chromosomal rearrangements in cancer samples at a high resolution. Using open-source software tools such as HiGlass, contact matrices enable the quick visualization of such large structural variants

Contents & Storage

  • TE Buffer pH 8.0
  • 10x Wash Buffer
  • TWB Solution
  • 2x NTB Solution
  • LWB Solution
  • NWB Solution
  • Chromatin Capture Beads
  • 10x Crosslink Reversal Buffer
  • Strepavidin Beads
  • 10x RBC Lysis Buffer
  • 20% SDS
  • 10% Triton X-100
  • 100 mM MnCl2
  • 0.5 M EDTA
  • Nuclease Enzyme Mix
  • 10x Nuclease Digest Buffer
  • End Polishing Enzyme Buffer
  • End Polishing Enzyme Mix
  • Intra-Aggregate Ligation Buffer
  • Intra-Aggregate Ligation Enzyme Mix
  • T4 DNA Ligase
  • Bridge
  • 5x Bridge Ligation Buffer
  • 250 mM DTT
  • HotStart PCR Ready Mix
  • Proteinase K

Documents

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