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Catalog #: 21007

Dovetail® HiChIP MNase Kit

The Dovetail HiChIP MNase Kit combines the benefits of ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Query protein-directed chromatin conformation mediated by specific proteins of interest and see your ChIP-seq data in a whole new dimension.
Ideal For:
Targeted 3D Topology
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HiChIP MNase Kit Video Overview

NEED CONTENT & VIDEO HERE

Life after ChIP-seq: genome conformation enhances our view of the regulatory landscape

Our Head of Customer Success, Dr. Myriam Elkhawand, will discuss how Dovetail® HiChIP data enables gene regulation to be understood at a deeper level. During this webinar you'll learn:

  • The role of genome conformation in gene regulation
  • Why topological interactions paint a more complete picture of gene regulation
  • How topology can be used in development, gene regulation, and disease studies
  • How to combine the 3-Dimensional data of Hi-C with ChIP-seq
WATCH VIDEO

See Your ChIP-seq Data in a Whole New Dimension

Genomic interactions matter. With ChIP-seq, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.

HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.

Get Nucleosome-Level Resolution…

Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).

…or 3D data at a fraction of the cost

Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.

Simple, Reproducible Workflow in an Easy-to-Use Kit

Go from sample to a sequence in just 2.5 days. Dovetail Genomics kits are ready-to-use out of the box so you don’t need to spend time optimizing your protocol or qualifying reagents. A novel dual-crosslinking approach delivers unmatched capture of long-range interactions and library complexity so you can analyze chromatin interactions from nucleosomes to megabases. Plus, using MNase eliminates the need to sonicate your sample, removing one of the biggest sources of experimental variability. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Customer Testimonials

"The quality of service from the Dovetail® scientific support team is one of the best we have experienced from vendors. They show a great deal of technical knowledge and a genuine care in supporting our efforts to prepare high quality proximity libraries using Dovetail® kits."
Dr. Orlando Contreras-López – Research Scientist at NGI Stockholm – SciLifeLab

Validated Antibodies

Dovetail Genomics has validated the following commercial antibodies so you can hit the ground running.
Dovetail HiChIP
Validated Antibody
Supplier
Part Number
IgG
Cell Signaling
2729
CTCF
Cell Signaling
3418
H3K4ac
Active Motif
39381
H3K4me3
Cell Signaling
9751
H3K14ac
Cell Signaling
7627
H3K27
Cell Signaling
8173
H3K27me3
Cell Signaling
9733
Dovetail HiChIP
Validated Antibody
Supplier
Part Number
H3K36me3
Cell Signaling
4909
Oct4
Cell Signaling
2890
Sox2
Cell Signaling
23064
Klf4
Abcam
ab106629
Nanog
Abcam
ab21624
PolII
Abcam
ab26721

Associated Dovetail® Kits

Dovetail® HiChIP MNase Kit

Dovetail® Library Module for Illumina

Dovetail® Dual Index Primer Set #1 for Illumina

Specifications

Reactions
8
Validated Samples
Mammalian cells
Applications
Protein directed chromatin conformation, gene-regulation & discovery of novel enhancers and super-enhancers
Module 1 of 2 – storage
2°C to 8°C
Module 1 of 2 – content
TE Buffer, pH 8.0
10x Wash Buffer
TWB Solution
2x NTB Solution
NWB Solution
Protein A/G Beads
10x Crosslink Reversal Buffer
Streptavidin Beads
10x RBC Lysis Buffer
20% SDS
Technique
Hi-C, ChIP-seq
Labeling
Research Use Only
Module 2 of 2 – storage
-30°C to -10°C
Module 2 of 2 – content
MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
Bridge
5x Bridge Ligation Buffer
T4 DNA Ligase
Proteinase K
250 mM DTT
HotStart PCR Ready Mix

Resources

Webinars
Product Literature
Manuals And Guides
Scientific Literature
Data Processing
How to Cite
Introduction to HiChIP MNase Technology
Life After ChIP-Seq: Genome Conformation Enhances Our View of the Regulatory Landscape
Next-gen epigenetics to understand why we age
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