Targeted Enrichment Panels

Catalogue #: 25013 (Human), 25014 (Mouse)

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What is Target Enrichment?

Next-generation sequencing (NGS) is an extremely powerful tool for capturing genome-wide genetic information. However, more narrowly focused approaches may be more suitable for many situations where the hypothesis is clearly and more narrowly defined, reducing overall study costs. This can be accomplished by hybridizing the sequencing library to capture probes that target pre-selected genomic regions and ‘pulling down’ the captured duplexes prior to sequencing, thereby enriching your library for genomic regions of interest. The result is a reduction in sequencing required to meet the sequence coverage goal for your targeted regions.

Targeted Enrichment Applied To Proximity Ligation Libraries

Targeted enrichment can be combined with proximity ligation libraries and is often referred to as capture Hi-C. However, any library bias must be considered during the enrichment panel design process. For instance, when working with traditional restriction enzyme based Hi-C libraries, unevenly distributed restriction sites result in coverage bias in the final library. Fortunately, due to the use of sequence independent nucleases, this source of bias is eliminated for Dovetail® Micro-C and Omni-C® libraries and standard design principles can be employed. While many commercially available targeted enrichment panels are compatible with our kits, many are not designed with the study of chromatin topology in mind. For this reason, Dovetail Genomics has developed the Dovetail® Pan Promoter Enrichment Panels for the study of chromatin topology anchored at gene promoter sites.

Dovetail® Pan Promoter Enrichment Panels

Dovetail® Pan Promoter Enrichment Panels target over 84,000 and 47,000 for human and mouse promoter sequences respectively offering comprehensive coverage of the promoter landscape. This inclusive design offers a genome-wide view of interactions anchored at known promoter sites enabling the detection of promoter specific interactions such as promoter-enhancer and promoter-promoter contacts.

Benefits

Comprehensive Coverage of Human or Mouse Promoter Regions

Covering greater than 98% of human and mouse promoter regions, Dovetail® Pan Promoter Enrichment panels are perfect for probing known and discovering unknown distal regulatory elements interacting with known promoters. The human design covers over 80,000 promoters associated with more than 27,000 coding and non-coding genes, while the mouse design covers over 47,000 promoters and 24,000 coding and non-coding genes..

Over 90% Less Sequencing Required Compared to Standard Hi-C

Standard Hi-C methods enable detection of promoter interactions through the calling of chromatin loops at high resolution (typically 5kb or greater). To achieve sufficient read depth to support these calls, sequencing depths in excess of 1 billion reads are required. Dovetail® Pan Promoter Enrichment Panels reduce sequencing 10-fold without compromising coverage over promoter regions.

High Enrichment Reproducibility

Within each panel, enrichment probes are balanced, and enrichment conditions optimized for consistent experiment to experiment results.

Improved Signal to Noise for High Resolution View of Promoter Specific Interactions

Targeted approaches reduce overall library complexity, both increasing representation of contacts of interest and reducing overall background signal. The result is more efficient use of sequence and greater evidence of contacts within regions of interest.

Easily Integrate Into Your Existing Workflow

Since hybridization capture is performed on the final proximity ligation libraries immediately prior to sequencing, there are no changes to the upfront proximity ligation workflow. In fact, existing Dovetail® Omni-C® and Micro-C libraries can be target enriched without the need to create new libraries for this purpose.

Specifications

Reactions

4

Validated Samples

Human or Mouse

Technique

Hi-C

Labeling

Research Use Only

Application(s)

Chromatin conformation analysis

Contents & Storage

Module 1 of 3: Dovetail® Target Enrichment Module

Store 2°C to 8°C

  • Streptavidin Binding Beads
  • DNA Purification Beads
  • Binding Buffer
  • Wash Buffer 1
  • Wash Buffer 2

Module 2 of 3: Dovetail® Hybridization Module

Store -25°C to -15°C

  • Universal Blockers
  • Blocker Solution
  • Hybridization Mix
  • Hybridization Enhancer
  • Amplification Primers
  • Capture Amplification Mix

Module 3 of 3: Dovetail® Promoter Enrichment Panel

Store -25°C to -15°C

  • Pan Promoter Panel

Documents

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