Comprehensive Coverage of Human or Mouse Promoter Regions
Covering greater than 98% of human and mouse promoter regions, Dovetail® Pan Promoter Enrichment panels are perfect for probing known and discovering unknown distal regulatory elements interacting with known promoters. The human design covers over 80,000 promoters associated with more than 27,000 coding and non-coding genes, while the mouse design covers over 47,000 promoters and 24,000 coding and non-coding genes..
Over 90% Less Sequencing Required Compared to Standard Hi-C
Standard Hi-C methods enable detection of promoter interactions through the calling of chromatin loops at high resolution (typically 5kb or greater). To achieve sufficient read depth to support these calls, sequencing depths in excess of 1 billion reads are required. Dovetail® Pan Promoter Enrichment Panels reduce sequencing 10-fold without compromising coverage over promoter regions.
High Enrichment Reproducibility
Within each panel, enrichment probes are balanced, and enrichment conditions optimized for consistent experiment to experiment results.
Improved Signal to Noise for High Resolution View of Promoter Specific Interactions
Targeted approaches reduce overall library complexity, both increasing representation of contacts of interest and reducing overall background signal. The result is more efficient use of sequence and greater evidence of contacts within regions of interest.
Easily Integrate Into Your Existing Workflow
Since hybridization capture is performed on the final proximity ligation libraries immediately prior to sequencing, there are no changes to the upfront proximity ligation workflow. In fact, existing Dovetail® Omni-C® and Micro-C libraries can be target enriched without the need to create new libraries for this purpose.