Welcome to our Testimonials page! This collection of customer testimonials spans over 8 years of exciting de novo assembly projects. You will see reference made to three generations of proximity ligation technology: Chicago™, Dovetail Hi-C™, and Omni-C™. Check back for new additions and many thanks to all those who provided us with a testimonial! We’d welcome your testimonial! Send us a message using the CONTACT US button above.
I have received a Dovetail Genome Assembly Award for the genome of a diploid species of Australian burrowing frog Neobatrachus pictus at the end of last year. Working with Dovetail on this project has been very efficient, after sending them tissue material both Dovetail and Chicago libraries has been done in a matter of weeks. The resulted HiRise assembly improved the scaffold N50 from 40 kb to 227.9 Mb, which is simply amazing and makes my further population genomics research on Neobatrachus genus finally possible.
Dovetail was very supportive and accommodating in talking me through the issues and sequencing another sample (from a single batch of embryos) that gave great results. We are currently in discussion re the next project.
Dovetail went above and beyond in helping us put together a contiguous genome, positioning us to discover the genetic basis for developmental evolution.
I am very satisfied with Dovetail’s service – we obtained an excellent genome assembly in a very short timeframe. This will allow us to better understand the molecular basis of pH regulation in marine invertebrates such as Hediste diversicolor, which is important in predicting responses to ocean acidification in future climates.
We are extremely satisfied with Dovetail’s work. They conducted scaffolding on 2 cricket genomes (Acheta domesticus and Grylloides signallus) for our exciting cutting edge DARPA (military) funded research project (SBIR Phase II grant contract) to generate genetic resources and produce transgenic edible insects (crickets and mealworms) that are higher in nutrients such as vitamin A and protein as well as being virus / disease resistant.
After struggling for years to assemble a high quality genome for the harlequin beetle riding pseudoscorpion using Illumina short-read sequencing, mate-pair libraries and eventually Nanopore long-read sequencing, we turned to Dovetail and started from scratch. After receiving considerable personal attention from business director Mark Daly and scientific director Shaune Hill, we chose PacBio Sequel II sequencing and HiRise scaffolding. The results are nothing short of spectacular, with 90% of the assembly (2.6 Gbp) contained in 24 scaffolds which corresponds exactly to the haploid number of chromosomes for the species. We were also able to confirm that the third largest scaffold, predicted to be the X chromosome based on size in the karyotype, bears an X-linked hypervariable microsatellite locus confirmed through DNA typing of pedigrees. In short, in a period of just a few months, Dovetail delivered a truly chromosomal level assembly that is a pleasure to behold.
We did the final scaffolding through Chicago and Hi-C libraries in Dovetail, and everything went very well. In our case, we provided an assembly of about 38.5 kb of N50, and we obtained one at the chromosomal-scale level. This assembly will allow us to carry out comparative and population genomic studies in multi-gene families. Among others, we are working on the major chemosensory system gene families.
Our experience with Dovetail Genomics has been entirely positive. Brenda Oppert and I incorporated Dovetail technology early in our genome projects and now have six assemblies ranging from 200 Mb to 2 Gb. We did our own PB assemblies with CANU, with 27-145x coverage, and Dovetail provided scaffolding with Chicago and HiC in HiRise providing N50 values from 13 to 222 Mb, and BUSCO scores from 95-99% after scaffolding. These results far exceeded our expectations, and we are in the process of publication of all six assemblies. Our project managers were professional and communicated in a timely manner, and all Dovetail personnel ensured the success of our projects. Now that Dovetail is offering annotation services, we are planning to incorporate that into our future projects, providing a one stop shop for complete genome analysis.
We had a matching grant from Dovetail Genomics and since then I have worked with them closely. The experience was superb! The Dovetail team was always there to answer our questions. After the first HiC+ Chicago, we went back and did additional 10X genomics sequencing. We asked Dovetail to use the additional sequences to re-build the assembly and they did without charging us any additional fee! I will not hesitate to recommend the company to anyone interested in chromosome level assembly.
Overall I have been very pleased and impressed with the service and outcomes of these projects. I have been a part of other genome assembly projects like this one in the past and can say this experience with Dovetail has been fantastic — very timely, responsive, and great quality of work.
With Dovetail Hi-Rise (Chicago and Hi-C), this complex heterozygous genome assembly was a breeze! Now there are better genomic resources to fight this invasive disease to save citrus production.
Having Dovetail complete the assembly and annotation gave my lab’s research a big boost. We have used the assembly for exciting population genomics work of this invasive species and have also used the annotation to begin to design CRISPRs.
We have done a few assemblies with Dovetail Genomics, and we’re very happy with the results. They have proved to be real experts in achieving chromosome-scale assemblies even from species with highly repetitive genomes. Their customer service is also excellent!
–Wirulda Pootakham, The National Center for Genetic Engineering and Biotechnology (BIOTEC)
