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Check out what others are saying about Dovetail!

Welcome to our Testimonials page! This collection of customer testimonials spans over 8 years of exciting de novo assembly projects. You will see reference made to three generations of proximity ligation technology: Chicago™, Dovetail Hi-C™, and Omni-C™. Check back for new additions and many thanks to all those who provided us with a testimonial! We’d welcome your testimonial! Send us a message using the CONTACT US button above.

ANIMALS

PLANTS

Animals

Painted burrowing frog

(Neobatrachus pictus)

Polina Novikova, Ghent University

I have received a Dovetail Genome Assembly Award for the genome of a diploid species of Australian burrowing frog Neobatrachus pictus at the end of last year. Working with Dovetail on this project has been very efficient, after sending them tissue material both Dovetail and Chicago libraries has been done in a matter of weeks. The resulted HiRise assembly improved the scaffold N50 from 40 kb to 227.9 Mb, which is simply amazing and makes my further population genomics research on Neobatrachus genus finally possible.

–Polina Novikova, Ghent University

Leech

(Helobdella austinensis)

David Weisblat, UC Berkeley

Dovetail was very supportive and accommodating in talking me through the issues and sequencing another sample (from a single batch of embryos) that gave great results. We are currently in discussion re the next project.

–David Weisblat, UC Berkeley

Polychaete

(Streblospio benedicti)

Matthew Rockman, New York University

Dovetail went above and beyond in helping us put together a contiguous genome, positioning us to discover the genetic basis for developmental evolution.

–Matthew Rockman, New York University

Ragworm

(Nereis diversicolor)

Katharina Wollenberg Valero, University of Hull

I am very satisfied with Dovetail’s service – we obtained an excellent genome assembly in a very short timeframe. This will allow us to better understand the molecular basis of pH regulation in marine invertebrates such as Hediste diversicolor, which is important in predicting responses to ocean acidification in future climates.

–Katharina Wollenberg Valero, University of Hull

Banded cricket

(Gryllodes sigillatus)

Aaron Dossey, All Things Bugs

We are extremely satisfied with Dovetail’s work. They conducted scaffolding on 2 cricket genomes (Acheta domesticus and Grylloides signallus) for our exciting cutting edge DARPA (military) funded research project (SBIR Phase II grant contract) to generate genetic resources and produce transgenic edible insects (crickets and mealworms) that are higher in nutrients such as vitamin A and protein as well as being virus / disease resistant.

–Aaron Dossey, All Things Bugs

Pseudoscorpion

(Cordylochernes scorpioides)

David Zeh, UNR

After struggling for years to assemble a high quality genome for the harlequin beetle riding pseudoscorpion using Illumina short-read sequencing, mate-pair libraries and eventually Nanopore long-read sequencing, we turned to Dovetail and started from scratch. After receiving considerable personal attention from business director Mark Daly and scientific director Shaune Hill, we chose PacBio Sequel II sequencing and HiRise scaffolding. The results are nothing short of spectacular, with 90% of the assembly (2.6 Gbp) contained in 24 scaffolds which corresponds exactly to the haploid number of chromosomes for the species. We were also able to confirm that the third largest scaffold, predicted to be the X chromosome based on size in the karyotype, bears an X-linked hypervariable microsatellite locus confirmed through DNA typing of pedigrees. In short, in a period of just a few months, Dovetail delivered a truly chromosomal level assembly that is a pleasure to behold.

–David Zeh, UNR

Woodlouse spider

(Dysdera crocata)

Julio Rozas, Univ. of Barcelona

We did the final scaffolding through Chicago and Hi-C libraries in Dovetail, and everything went very well. In our case, we provided an assembly of about 38.5 kb of N50, and we obtained one at the chromosomal-scale level. This assembly will allow us to carry out comparative and population genomic studies in multi-gene families. Among others, we are working on the major chemosensory system gene families.

–Julio Rozas, Univ. of Barcelona

Insect pests

(Curculionoidea)

Marcé D. Lorenzen, NC State University

Our experience with Dovetail Genomics has been entirely positive. Brenda Oppert and I incorporated Dovetail technology early in our genome projects and now have six assemblies ranging from 200 Mb to 2 Gb. We did our own PB assemblies with CANU, with 27-145x coverage, and Dovetail provided scaffolding with Chicago and HiC in HiRise providing N50 values from 13 to 222 Mb, and BUSCO scores from 95-99% after scaffolding. These results far exceeded our expectations, and we are in the process of publication of all six assemblies. Our project managers were professional and communicated in a timely manner, and all Dovetail personnel ensured the success of our projects. Now that Dovetail is offering annotation services, we are planning to incorporate that into our future projects, providing a one stop shop for complete genome analysis.

–Marcé D. Lorenzen, NC State University

Squash bee

(Eucera (Peponapis) pruinosa)

Margarita López-Uribe, Penn State

This is very exciting! Thomas, thank you for sharing this final assembly with us! The quality of this genome is better than I ever dreamed of 🙂

–Margarita López-Uribe, Penn State

Deer tick

(Ixodes scapularis)

Monika Gulia-Nuss, UNR

We had a matching grant from Dovetail Genomics and since then I have worked with them closely. The experience was superb! The Dovetail team was always there to answer our questions. After the first HiC+ Chicago, we went back and did additional 10X genomics sequencing. We asked Dovetail to use the additional sequences to re-build the assembly and they did without charging us any additional fee! I will not hesitate to recommend the company to anyone interested in chromosome level assembly.

–Monika Gulia-Nuss, UNR

Dung beetle

(Onthophagus taurus)

Phillip Davidson, Indiana University

Overall I have been very pleased and impressed with the service and outcomes of these projects. I have been a part of other genome assembly projects like this one in the past and can say this experience with Dovetail has been fantastic — very timely, responsive, and great quality of work.

–Phillip Davidson, Indiana University

Asian citrus psyllid

(Diaphorina citri)

Preshant Hosmani, Boyce Thompson Institute

With Dovetail Hi-Rise (Chicago and Hi-C), this complex heterozygous genome assembly was a breeze! Now there are better genomic resources to fight this invasive disease to save citrus production.

–Preshant Hosmani, Boyce Thompson Institute

African fig fly

(Zaprionus indianus)

Priscilla Erickson, Univ. of Richmond

Having Dovetail complete the assembly and annotation gave my lab’s research a big boost. We have used the assembly for exciting population genomics work of this invasive species and have also used the annotation to begin to design CRISPRs.

–Priscilla Erickson, Univ. of Richmond

Giant tiger prawn

(Penaeus monodon)

Wirulda Pootakham, BIOTEC

We have done a few assemblies with Dovetail Genomics, and we’re very happy with the results. They have proved to be real experts in achieving chromosome-scale assemblies even from species with highly repetitive genomes. Their customer service is also excellent!

–Wirulda Pootakham, The National Center for Genetic Engineering and Biotechnology (BIOTEC)

Petiole gall psyllid

(Pachypsylla venusta)

Yiyuan Li, Univ. of Texas, Austin

Genome assembly is one of the most fundamental and difficult tasks in comparative genomics. We often get no chromosome information from genome assembly. The chromosome-level assemblies of aphid and psyllid using Dovetail HiC and Chicago provided tremendous information of chromosomal locations of genes. These genomes jump started our project and greatly improved our understanding of chromosome evolution of hemipterans.

–Yiyuan Li, Univ. of Texas, Austin

Myrtle warbler

(Setophaga coronata)

David Toews, Penn State

Dr. Brelsford and I have been working on warbler genomics together for nearly a decade now! Dovetail’s Chicago and Hi-C libraries are finally allowing us to put together our small scaffolds from our first generation assembly towards a near chromosome-level genome. We are excited to see what advances this will bring us in our understanding of avian genome evolution!

–David Toews, Pennsylvania State University

Broad-tailed hummingbird

(Selasphorus platycercus)

Nicolas Alexandre, UC Berkeley

Our use of a combination of HiC and Chicago improved our PacBio assembly considerably. N50 increased from 1.4 Mb to 7.2 Mb with an almost tenfold reduction in contig number between assemblies (contigs >= 1000 bp = 5605, 483). This high quality assembly will allow us to link medium to large structural variants to morphological differences in hummingbird birds.

–Nicolas Alexandre, UC, Berkeley

Mallard

(Anas platyrhynchos)

Phil Lavretsky, Univ. of Texas, El Paso

Working with the folks at Dovetail has been a pleasure from the time we started extractions to today’s final analyses. Even after a couple years since completing sequencing, Dovetail continues to be responsive and providing expertise every step of the way. Importantly, the three duck genomes they provided have allowed my team at the University of Texas at El Paso to tackle important evolutionary and conservation questions. I could not be happier with service or product that Dovetail provided.

–Phil Lavretsky, Univ. of Texas, El Paso

Brown-capped rosy finch

(Leucosticte australis)

Scott Taylor , Univ. of Colorado, Boulder

Having a reference genome from Dovetail has been great. The large scaffold size allows us to jump straight in to analyses. From detecting peaks of divergence to describing structural variants, such a high quality reference has been essential for all of our work on non-model organisms

–Scott Taylor , University of Colorado, Boulder

White-crowned sparrow

(Zonotrichia leucophrys)

Jacqueline Smith, Roslin Institute

Our experience with Dovetail was absolutely fantastic. Excellent communication and service throughout. We have received three amazing assemblies for different sparrow species, based on PacBio sequencing and Omni-C scaffolding. This will allow our investigation of the genetics underlying seasonal breeding patterns and resilience to environmental change. Highly recommended!

–Jacqueline Smith, Roslin Institute

Longnose gar

(Lepisosteus osseus)

Alex Dornburg, Friends of the Museum of Natural Sciences

While it’ll take us a few months to make it all the way our analyses, we are delighted with both the quality of the data, and the speed at which you got it to us.

–Alex Dornburg, Friends of the Museum of Natural Sciences

Anglerfish

(Lophius piscatorius)

Arseny Dubin, Nord University

It was a pleasure to work with you and Dovetail! It was certainly an “expectation-exceeding experience”! And I will definitely recommend Dovetail to my colleagues who are starting another fish genome project! The assembly stats are fantastic!! It is better than the previous assembly and has no parasite sequences (almost).

–Arseny Dubin, Nord University

Atlantic bluefin tuna

(Thunnus thynnus)

Barbara Block, Stanford University

We are very excited to have received fully phased genomes for both of our haplotypes of Atlantic bluefin tuna. We are thrilled to have worked with Dovetail as an early access customer.

–Barbara Block, Stanford University

Fugu fish

(Tetraodontidae)

Hyun Park , Korea University

Through various kinds of fish genome research, Dovetail Hi-C kit and HiRise assembly showed highest performance. When scaffoldings were performed using Dovetail Hi-C kit on PacBio long reads draft assembly, it showed chromosome level scaffolding in all cases. As one example, we obtained the highest quality of fugu genome reported so far, the scaffold N50 improved from 3.3 Mb to 15.2 Mb and resulted in 22 chromosomes which is the chromosome level assembly.

–Hyun Park, Korea University

Spined loach

(Cobitis taenia)

Karel Janko, Czech Academy of Sciences

From the first glance: that looks awesome! We seem to be at the chromosomal level: C. taenia indeed has 24 chromosomes, which seem to be met by your analysis, if I understand it right. We will give it a look at greater detail, but I just wanted to thank you and all your team for this great achievement.

–Karel Janko, Czech Academy of Sciences

Hammerhead shark

(Sphyrna mokarran)

Mahmood Shivji, Nova Southeastern University

We are working with Dovetail to improve our independently derived, shark hybrid (short red-long-read) assembly. While this work is still ongoing, the Dovetail customer service team has been very helpful and communicative with us during each step.

–Mahmood Shivji, Nova Southeastern University

Fish

Stephen Sabatino, University of Porto

This is really amazing news and I’m very excited about the results. Many, many thanks for working with me to get it done. I did a synteny analysis of the last version of the assembly using a few good reference genomes and it further confirmed the quality of the results. Karotype analysis suggests the sardine has around 24 chromosomes and that fits well with the assembly that was generated. Since this genome assembly is even more complete, I expect this version to be even beter with respect to the annotation, which was already in good shape.

–Stephen Sabatino, University of Porto

Bangai cardinalfish

(Pterapogon kauderni)

Giacomo Bernardi, University of California, Santa Cruz

We are thrilled to be finally able to generate a genome assembly for this critically endangered fish.The genome assembly will be a critical tool that we will put to use immediately to measure population diversity, heterozygosity and other metrics that will help us in our quest to save this species.

–Giacomo Bernardi, University of California, Santa Cruz

Midas cichlid

(Amphilophus citrinellus)

Axel Meyer, University of Konstanz

We really, really liked our experience with Dovetail and are extremely pleased with the data obtained from you! The improvement in N50 length makes this method – in conjunction with sufficient sequencing depth and variation in library sizes – attractive to projects aiming at developing high quality reference genomes.

–Axel Meyer, University of Konstanz

Lancelet

(Amphioxus)

Jia-Xing Yue, Academia Sinica

Great! I briefly checked the report and the N90 scaffold count matches well with the expect number of chromosomes in a haploid genome of our sequenced animal. I will perform more detailed check in the following few weeks.

–Jia-Xing Yue, Academia Sinica

Tiger

(Panthera tigris)

Ellie Armstrong, Stanford University

Dovetail’s HiC and Chicago products, and HiRise software have gone above and beyond our expectations for a variety of organisms and projects in our group. We have been able to substantially improve complex arthropod genome assemblies over 100-fold and easily scaffold mammalian assemblies to the chromosome level with their help. We would highly recommend their services to anyone looking for a reliable and responsive team to take your assemblies to the next level. Further, their new HiC kits are easy to use and provide an affordable way to upgrade multiple assemblies–an important addition for the species of conservation concern that we work with!

–Ellie Armstrong, Stanford University

Eastern quoll

(Dasyurus viverrinus)

Charles Feigin , Princeton University

For our conservation genomics project, we provided Dovetail with a precious and limited sample from our species of interest. We were blown away by the completeness and of the chromosome-scale assembly they produced using HiFi, Omni-C, and the HiRise pipeline. With an assembly drastically exceeding Vertebrate Genomes Project standards in hand, we can put all our focus on the downstream science.

–Charles Feigin, Princeton University

Leaf-eared mouse

(Phyllotis vaccarum)

Jay Storz, University of Nebraska

Our new genome assembly for the world’s highest-dwelling mammal turned out great! This will be a great resource for studies of hypoxia adaptation.

–Jay Storz, University of Nebraska

Black rat

(Rattus rattus)

Johannes Krause, Max Planck Institute

With the Dovetail genome assembly pipeline, we generated the first genome assembly of the black rat, a well-known commensal species of human. The scaffold N50 reached over 100 Mb and each of the 19 autosomes are mostly reconstructed into one large scaffold. Such chromosome-level completeness is astonishing for a de novo assembly. This genome works as a great reference for our popula’on genomic study of the black rat and will be an important resource for further studies.

–Johannes Krause, Max Planck Institute

Pacific pocket mouse

(Perognathus longimembris pacificus)

Aryn Wilder, San Diego Zoo Global

Having a high-quality reference genome is an enormous conservation tool, providing a critical foundation for measuring extinction risk and designing optimal strategies for conserving species. This genome map will help inform our work to save this species.

–Aryn Wilder, San Diego Zoo Global

Alpaca

(Vicugna pacos)

Kylie Munyard, Curtin University

The upgrade from a (very patchy) version 2 to “almost chromosome” version 3 of the alpaca genome was greatly assisted by the addition of Dovetail Chicago results. Using the new genome, my team was able to confirm a causative mutation for classic grey in alpacas.

–Kylie Munyard, Curtin University

Caribou

(Rangifer tarandus)

Rebekah Horn, Trent University

Dovetail Genomics assembled our high-quality caribou reference genome using Chicago libraries and HiRise assembly. The genome has been invaluable in our current research (@LandSciTech @clycaon @BeckySTaylor), and we now have over 50 re-sequenced caribou genomes (with more on the way) which were aligned to the Dovetail genome, which is leading to leaps forward in our knowledge of caribou evolution and will help with conservation strategies!

–Rebekah Horn, Trent University

Visayan warty pig

(Sus cebifrons)

Martien Groenen, Wageningen University

We are very pleased with the result of the assembly. It really is beyond our expectations. The first analyses have already shown some very nice rearrangements compared to the genomes of other closely related species. I also am very positive how you handled the whole process and kept us informed about the status during the different steps of the assembly.

–Martien Groenen, Wageningen University

Gray fox

(Urocyon cinereoargenteus)

Alexis M. Mychajliw, Middlebury College

I see gray fox tracks and scat daily on our college campus, but we don’t even know when or how this species colonized the northeast, let alone how climate change and increasing coyote and domestic dog populations may shape its future ecology. The genome generated with Cantata will allow us to address these questions about the species’ biology and provide tools for teaching and engagement with the public.

–Alexis M. Mychajliw, Middlebury College

Narwhal

(Monodon monoceros)

Martin Nweeia, Harvard School of Dental Medicine

The Dovetail team brings excellence to the almost every platform of genetic research. They are knowledgeable, resourceful, pleasant and hardworking navigating results beyond expectation. The Narwhal Genome Initiative has benefited from their ongoing support and input to establish a reference genome for the narwhal. They deserve the accolades that place them as an industry standard on the world stage.

–Martin Nweeia, Harvard School of Dental Medicine

Humpback whale

(Megaptera novaeangliae)

Giugi Carminati, Center for Cetacean Research and Conservation

I am beyond grateful to be partnering with Cantata Bio and Revive & Restore to develop a first-of-its-kind genome for the humpback whale. Using a sample collected by the Center for Cetacean Research and Conservation, we will open the door for a whole new avenue of research and inquiry.

–Giugi Carminati, Center for Cetacean Research and Conservation

Sea otter

(Enhydra lutris)

Annabel Beichman, University of Washington

We sequenced, assembled, and annotated the southern sea otter reference genome, which allowed us to carry out comparative genomics analyses and begin to assess levels of genetic diversity. Our comprehensive approach shows the role of a reference genome in enabling wide-ranging analyses and demonstrates how demographic inference from genomic data, coupled with simulations, allows assessment of extinction risk and different models of recovery.

–Annabel Beichman, University of Washington

Vampire bat

(Desmodus rotundus)

Tom Gilbert, University of Copenhagen

The first genome we assembled was pretty good for a mammal genome, but nothing compared to your assembly of course! We were simply blown away by the N50 and N90. In short the benefits were huge compared to the cost.

–Tom Gilbert, University of Copenhagen

Northern bat

(Eptesicus nilssonii)

Veronika Laine, Finnish Museum of Natural History

With the help of Dovetail and their Genome Assembly award the reference genome of the northern bat (Eptesicus nilssonii) is ready. Northern bat is a widespread bat species in Eurasia and can be even seen in the Arctic Circle. It is important species in studying adaptation to northern latitudes and climate change. Thus, having this genome is essential. Dovetail did excellent job providing us good quality reference genome with gene annotation. Everything went smoothly and we are so excited to start working with the genome.

–Veronika Laine, Finnish Museum of Natural History

American alligator

(Alligator mississippiensis)

Edward Rice, UC Santa Cruz

The increased contiguity of (the Chicago assembly) vastly improved our ability to compute synteny between the chicken and alligator genomes, more than doubling the percentage of the genome covered by synteny blocks from 35% to 90% and increasing the sizes of synteny blocks, with 57 of the 90 synteny blocks over 10 Mb in length (Rice ES et al., 2017, Genome Research – adv online: doi:10.1101).

–Edward Rice, UC Santa Cruz

Sonoran desert tortoise

(Gopherus morafkai)

Greer Dolby, Arizona State University

We used Dovetail Chicago and HiRise services to improve a first-generation vertebrate reference genome and received an excellent product with chromosome-scale scaffolds. The contiguity of the assembly was superb and the work was done quickly and for a good price. We’ve since gone back to work with them on additional reference genomes and are excited to get working on analyzing these new resources!

–Greer Dolby, Arizona State University

Indian cobra

(Naja naja)

Kushal Suryamonhan, Genentech

We were truly amazed to see a near-chromosomal assembly of the highly venomous and iconic Indian cobra genome. This allowed us for the first time to identify the key venom genes responsible for the toxicity in the venom of this snake that leads to thousands of deaths each year! This would not have been possible without Dovetail Genomics. We look forward to sequencing many more genomes to empower genomic research!

–Kushal Suryamonhan, Genentech

Mourning gecko

(Lepidodactylus lugubris )

Thomas Lozito, University of Pittsburgh

Studying lizard tail regeneration, I often dreamed of having more lizard genomes to dissect the genetics of amniote tail regrowth, but genome sequencing always seemed outside of my budget and expertise. Working with Dovetail Genomics has changed how I view genomic science, and I now realize, with Dovetail’s help, so much is possible. I have been amazed at the highest quality of work at all steps of the Dovetail pipeline, and the produced genomes are undeniably superior. The Lepidodactylus lugubris genome has helped us develop this parthenogenetic lizard as a model of cell lineage tracing during blastema formation. I look forward to continuing working with Dovetail.

–Thomas Lozito, University of Pittsburgh

Brown anole

(Anolis sagrei)

Anthony Geneva, Drexel University

Chicago increased our N50 substantially, Dovetail Hi-C even more so. We ended up with a final assembly with N50 of 314Mb – highly, highly contiguous for an anole. We had a very, very complete assembly as well, we had only 2% of the genes missing from the vertebrate BUSCO dataset and the vast majority of genes were present in full-length and single-copy. We know in the history of Anolis that there’s been a complicated series of fusions and fissions. We were able to stitch together and show this process of fusions and fissions in the history of Anolis simply by having incredibly complete and contiguous assemblies and chromosome-scale scaffolds.

–Anthony Geneva, Drexel University

Giant squid

(Architeuthis sanctipauli)

Tom Gilbert, University of Copenhagen

The giant squid genome continually defeated us when using a range of other sequencing technologies. However when we turned to Dovetail, finally things took a major turn for the better, allowing us to finally get a decent assembly from it.

–Tom Gilbert, University of Copenhagen

Jellyfish

(Medusozoa)

Jerome Hui, The Chinese University of Hong Kong

Having bona fide high-quality genomes are always crucial for downstream analyses. The quality of the genomic scaffolds is more than simply getting a larger number in its N50, but also whether the contigs are correctly linked in the right places. Our past experiences working together with Dovetail have been great on this aspect.

–Jerome Hui, The Chinese University of Hong Kong

Starlet sea anemone

(Nematostella vectensis)

Bob Zimmermann, Universität Wien

Our post-Dovetail Genomics assembly was a tremendous improvement over our PacBio assembly. It was reassuring to have assembly experts at the helm to analyze the data.

–Bob Zimmermann, Universität Wien

Lace coral

(Pocillopora damicornis)

Nikki Traylor-Knowles, Rosenstiel School of Marine and Atmospheric Science

Dovetail genomics sequenced and assembled a high quality genome for the lace coral, Pocillopora damicornis, an important reef building coral in the Indo-Pacific. This resource has become incredibly important to furthering our understanding of coral reef healthy and conservation. The quality and quickness with which Dovetail delivered this genome set a high industry standard.

–Nikki Traylor-Knowles, Rosenstiel School of Marine and Atmospheric Science

Rice coral

(Montipora capitata)

Debashish Bhattacharya, Rutgers University

A putative chromosomal-level assembly of the Montipora capitata genome has been achieved using the Dovetail pipeline. This genome is being used to explore population divergence in the Hawaiian Archipelago, elucidate thermal stress pathways using multi-omics and reverse genetics, and understand the genetic basis of the fast-growing (opportunistic) phenotype observed in populations that inhabit Ulithi Atoll in Micronesia.

–Debashish Bhattacharya, Rutgers University

Sunflower sea star

(Pycnopodia helianthoides)

Lauren Schiebelhut , University of California, Merced

Dramatic declines (in Pycnopodia helianthoides numbers) and their community consequences have spurred the need for quick concerted actions to conserve the species and collect the information necessary to inform potential conservation interventions. With Revive & Restore, we are working to discover genomic variants associated with vulnerability to wasting and temperature stress. A high-quality genome assembly was a crucial first step in this effort and was provided by DTG through the R&R Wild Genomes Program. The assembly will be used to generate the genomic insights needed to help inform conservation decisions and increase success along two conservation trajectories: (1) captive breeding for potential out-planting and (2) assessing wild populations for potential translocation and other conservation actions.

–Lauren Schiebelhut , University of California, Merced

Ochre sea star

(Pisaster ochraceus)

Michael Dawson, University of California, Merced

So many ochre sea stars have died, it seemed unimaginable that there weren’t genomic signatures. But assembling a high quality reference genome to empower our population-level research was beyond our reach. We turned to Dovetail Genomics on the basis of a strong recommendation and yet still were surprised by their high standards, persistence, professionalism, and support. Dovetail delivered a substantially complete chromosome level assembly in a fraction of the time that greatly accelerated our research. We were so happy with the product and service that Dovetail is now sequencing and assembling our fourth genome, and we’ve come to see them as almost a part of the lab. Thank you Dovetail!

–Michael Dawson, University of California, Merced

Chilean sea urchin

(Tetrapygus niger)

Felipe Aguilera, Universidad de Concepción

Tetrapygus niger is a sea urchin species inhabiting Peruvian-Chilean waters with no genomic resources so far. Therefore, we aimed to sequence its genome with Dovetail Genomics and the genome assembly delivered met all our expectations and beyond. With this chromosome-level genome assembly in our hands, we are trying to establish T. niger as an emerging research organism from South America with the goal of positioning this species as a model system to investigate the evolution of cell types, developmental gene regulatory networks, deuterostome genome evolution, and animal adaptation to changing environments.

–Felipe Aguilera, Universidad de Concepción

Collector urchin

(Tripneustes gratilla)

Aki Jarl Laruson Cornell University, Floyd Reed, University of Hawaii at Manoa

We’ve been very lucky to work with Dovetail on the sequencing and assembly of the genome of the collector urchin Hawa`e maoli (Tripneustes gratilla). The sequencing and preliminary assembly had a great turnaround time, and the technicians responded promptly and helpfully to every question we had. This will be the first ever genome sequence of a Hawaiian echinoid. A reference genome of this quality is a boon for future marine macroinvertebrate research, and solidifies the importance of this species for future aquaculture endeavors.

–Aki Jarl Laruson, Cornell University & Floyd Reed, University of Hawaii at Manoa

Sea snail

(Crepidula atrasolea)

Deirdre Lyons, Scripps Institute of Oceanography

Dovetail Genomics has worked with us (me and Prof Jon Henry, University of Illinois Urbana-Champaign) extensively on three “non-model” genome projects and we’ve always been impressed with their helpfulness and dedication to delivering us quality data!

–Deirdre Lyons, Scripps Institute of Oceanography

Cone snail

(Conidae)

Rafael Zardoya, Museo Nacional de Ciencias Naturales

Our snail genome project resulted more challenging that initially thought. Yet, people at Dovetail never surrendered and found the way to succeed. The high-quality chromosme-level assembly has fully met our expectations and is now rendering very interesting results.

–Rafael Zardoya, Museo Nacional de Ciencias Naturales

Bermuda land snail

(Poecilozonites bermudensis)

David Polly, Indiana University

For our collaboration with Mark Outerbridge, Stevie Winingear, Ann Stone, and the Chester Zoo, we needed a full genome from this endangered snail to study conservation genetics and evolutionary history, but there are no close relatives with a full sequence, plus snails are notoriously difficult to sequence because of their mucoids. Dovetail’s OmniC technology made a chromosome-scale scaffold possible.

–David Polly, Indiana University

Surf clam

(Bivalvia)

Max Glanz, Radial Therapeutics

The scaffolding worked incredibly well for us and I cannot even begin to express my sincerest thanks and appreciation to you and the Dovetail team for making this happen.

–Max Glanz, Radial Therapeutics

Blue mussel

(Mytilus edulis)

Tiago Hori, Atlantic Aquafarms

Highly heterozygous bivalve genomes are notoriously hard to assemble, even using long-read technologies. Dovetail’s Omni-C technology allowed us to assemble a high-quality chromosome-level genome for Mytilus edulis directly from a draft long-read assembly. This high-quality genome will enable us to study presence-absence variation in the appears to be a key element of the adaptability of the genus Mytilus and generate important genetic resources for these commercially important bivalves.

–Tiago Hori, Atlantic Aquafarms

Atlantic awning clam

(Solemya velum)

Vanessa Gonzalez, Smithsonian Institution

We generated a reference-quality genome Solemya velum (Bivalvia: Protobranchia) to allow us to test hypotheses about mollusc genome evolution and the symbiotic interactions in marine molluscs. 10X Genomics, PacBio Long-reads and Hi-C data was leveraged for molluscan genome assembly. Iterations and combinations of each data type have resulted in a 2.4 GB reference genome with a scaffold N50 of 200 MB and a BUSCO completeness score of 93.7%.

–Vanessa Gonzalez, Smithsonian Institution

Ocean quahog

(Arctica islandica)

Stephen Treaster, Harvard Medical School

We sent Dovetail tissue samples and they sent us a high quality genome, with prompt and expert technical support, at a great price. Can’t ask for anything else and we’re planning to use their services again. At the Harris lab (www.fishbonelab.org) this genome will enable comparative genomics and population genetics analyses to unravel the ocean quahog’s secrets of exceptional longevity, up to 507 years.

–Stephen Treaster, Harvard Medical School

True limpet

(Scurria scurra )

Pablo Saenz-Agudelo, Universidad Austral de Chile

We are very satisfied with the results of the assembly. This new high quality genome will help us take our study of adaptation to highly variable environments to a new level. We are still working on the analysis of the data, but definitely Dovetail met all our expectations, not only in terms of the quality of the data, but also because they provided a great service and support all through the process.

–Pablo Saenz-Agudelo, Universidad Austral de Chile

New world hookworm

(Necator americanus )

Erich Schwarz, Cornell University

Your previous work on Omni-C of the hookworm Necator americanus worked beautifully. Your documentation for processing reads was clear and actionable (https://omni-c.readthedocs.io/en/latest/index.html), and I was able to use SALSA2 (https://github.com/marbl/SALSA) with these processed reads to achieve scaffolds for six chromosomes (five of them perfect, with one-scaffold-per-chromosome; one of them with only a single break). Given the quality and efficacy of your results, my collaborator Prof. Adler Dillman (UC Riverside) and I would like to get price quotes from you for doing Omni-C on two more nematodes.

–Erich Schwarz, Cornell University

Flatworm

(Dugesia hepta )

Marta Riutort, Biodiversity Research Institute (IRBio)

We have a project to sequence the genome of three Dugesia flatwom species, animals with around 2 Gbp genomes, for developing diverse studies on their evolution. The only genomes published till now for this group belong to a species from a close genus Schmidtea mediterranea for which, until very recently, only a highly fragmented genome was available. For the first two species of our project, Dovetail using PacBio and HiRise have generated a chromosome level assembly, which seems to me nearly unbelievable. I thank Dovetail for their professionality and good results. With these genomes we will be able to perform studies on chromosomal reorganizations and the effects of long-term fissiparity.

–Marta Riutort, Biodiversity Research Institute (IRBio)

Plants

Pale flax

(Linum bienne)

Adrian Brennan, Durham University

Dovetail Genomics helped me in my research aim to generate de novo genome assemblies for the wild relatives of an important fibre and oilseed crop, flax. High quality genomes are becoming an essential starting point for genomics research and Dovetail Genomics has fueled my entry into this field to learn more about the genomic changes associated with the domestication of flax. The process was simple, starting with sending a sample of fresh material, and Dovetail Genomics did the rest, providing a quick service turn-around and additional support as necessary. I am very pleased with the results.

–Adrian Brennan, Durham University

Spinach

(Spinacia oleracea)

Ainong Shi, University of Arkansas

I would like to express my thanks to you for your excellent job to do whole genome sequencing in our spinach samples! We will use the spinach genome sequence as a reference in our spinach sequencing study. We are satisfied with your work and the results what you did.

–Ainong Shi, University of Arkansas

Manzanita

(Arctostaphylos glauca)

Amy Litt, University of California Riverside

That is extremely impressive, thanks! We could not be more excited.

-Amy Litt, University of California Riverside

Air plant

(Tillandsia leiboldiana )

Christian Lexer, University of Veterinary Medicine, Vienna

Wow this is fantastic, looks like we´re on track for a chromosome level assembly!

–Christian Lexer, University of Veterinary Medicine, Vienna

Wild peanut

(Arachis duranensis)

David Bertioli, University of Georgia

Dovetail Hi-C produced incredible scaffold ordering for our assemblies of the diploid ancestral species of peanut, Arachis duranensis and A. ipaensis. The new orderings made even clearer the incredibly close relationships between the genomes of these diploid species and the tetraploid crop.

–David Bertioli, University of Georgia

Buffalograss

(Bouteloua dactyloides)

David Huff, Pennsylvania State University

Thanks to everyone at Dovetail Genomics for the buffalograss genome! It is amazing and so are all of you too!

–David Huff, Pennsylvania State University

Kiwi

(Actinidia melanandra)

Elena Hilario, The New Zealand Inst. for Plant & Food Research Ltd.

Dovetail Genomics assembled a kiwifruit genotype for us to chromosome level and this assembly exhibits impressive synteny to existing whole genome sequences of other kiwifruit genotypes. We are very happy with the results achieved using Dovetail Chicago and Hi-C libraries and their HiRise assembly.

–Elena Hilario, The New Zealand Inst. for Plant & Food Research Ltd.

Cowslip

(Primula veris)

Giacomo Potente, University of Zurich

We are delighted by the results you provided to us! So thank to Dovetail, and thank you guys for managing the project so professionally! The paper presenting the chromosome-scale assembly of P. veris has been published in Molecular Biology and Evolution (https://doi.org/10.1093/molbev/msac035)

–Giacomo Potente, University of Zurich

Wild soybean

(Glycine soja)

Hon-Ming Lam, Chinese University of Hong Kong

Our soybean research team (@AoE_GSPEI) is very satisfied with the Dovetail Hi-C library and HiRise analysis pipelines. It was very helpful in putting thousands of scaffolds into high-quality chromosomes. We are excited by the discovery of differences between wild and cultivated soybeans using the resulting high-quality, chromosome-scale wild soybean genome.

–Hon-Ming Lam, Chinese University of Hong Kong

Plantain

(Musa)

Jaroslav Dolezel, Institute of Experimental Botany

I have been extremely happy with the service provided by Dovetail Genomics. The communication with them has always been very easy and, importantly, they are willing to help with difficult projects.

–Jaroslav Dolezel, Institute of Experimental Botany

Wild oat

(Avena eriantha)

Jeff Maughan, Brigham Young University

Dovetail successfully scaffolded the ancestral A and C genomes of oat from Sequel II contigs for our group (3+ Gb genomes). These are highly repetitive genomes, so I had my worries that they’d get to chromosome scale – but the resulting scaffolds looked great (7 complete chromosomes; telomere to telomere in many cases). Comparison to linkage maps confirmed the scaffolding with very few exceptions (which could have been a linkage mapping error). We more recently compared their Omni-C with their Hi-C methodologies – both produced chromosome level scaffolds of Bromus tectorum (cheatgrass) – another 3+ Gb genome). We are in the middle of comparing the two scaffolded genomes to see if we like one better than the other – but both look pretty good. Dovetail has always been easy to work with and we appreciate that they respond professionally to our concerns in a timely manner.

–Jeff Maughan, Brigham Young University

Northern wild rice

(Zizania palustris)

Jennifer Kimball, University of Minnesota

I’m very happy I chose to work with Dovetail Genomics to sequence the Zizania genome. They were friendly, knowledgeable, and very communicative when I had concerns, questions, or inquired about updates throughout the process. The Zizania genome sequence and assembly has already been vetted by several other professionals, who have commented on its high quality. This resource will continue to aid in the conservation of natural stands of Zizania as well as the dissection of the underlying genetic mechanisms of important agronomic traits associated with cultivated Zizania varieties.

–Jennifer Kimball, University of Minnesota

Wild sunflower

(Helianthus anomalus)

Loren Rieseberg, University of British Columbia

This is very impressive indeed! Those look like the 17 chromosomes we would expect. We will start looking at the assembly more in details and get back to you if we have any questions, but for now I would say this definitely exceeded expectations.

–Loren Rieseberg , University of British Columbia

Wild sunflower

(Helianthus argophyllus)

Marco Todesco, University of British Columbia

Our experience with Dovetail has been excellent (and) in all our projects, results consistently exceeded our expectations, and (Dovetail) has always been very flexible and supportive. In particular, we did two scaffolding projects with Dovetail, and in both cases got back very high quality, chromosome levels assemblies. The latter project was probably more impressive, since we started with a rather fragmented 10X linked-reads assembly of a highly heterozygous wild sunflower species (~5.6 Gbp genome, >80% TEs), and after Chicago and HiC ended up with entire chromosomes which lined up perfectly with our high-density genetic maps for that species.

–Marco Todesco, University of British Columbia

Annual ragweed

(Ambrosia artemisiifolia)

Mike Martin, Norwegian University of Science and Technology

Many thanks Jasmine & team. We are impressed by the increase in N50 aher scaffolding, it’s looking much better! We are planning to use this new assembly in our further analyses.

–Mike Martin, Norwegian University of Science and Technology

Goat’s foot

(Ipomoea biloba)

Naganeeswaran Sudalaimuthuasari, United Arab Emirates University

We are very much happy about the Ipomea biloba genome project with Dovetail. The final genome assembly is really good and the N50 value met our expectations.

–Naganeeswaran Sudalaimuthuasari, United Arab Emirates University

Lettuce

(Lactuca sativa)

Richard Michelmore, University of California, Davis

The Dovetail approach was able to accommodate the high repeat content of the lettuce genome and identified chimeric scaffolds that were missed by genetic analysis. Our resulting assembly was greatly improved in accuracy and contiguity and serves as the reference genome for the community.

–Richard Michelmore, University of California, Davis

Brittlebush

(Encelia farinosa)

Sonal Singhal, California State University, Dominguez Hills

Dovetail took what seemed like an impossible project — getting an assembly for a plant with a large, repetitive genome — and made it a reality. They collaborated with different bioinformatics groups & did additional analyses to make sure we got the best results possible.

–Sonal Singhal, California State University, Dominguez Hills

Milkweed

(Euphorbia peplus)

Tomasz Czechowski, University of York

I want to thank you and the rest of the Dovetail team for all of the work you have done for us in this project. I think we are all pleased with the quality of the data retrieved and your very professional attitude. It was a pleasure working with you. We might come back to you in the future in case we need more details for the publication of that genome assembly but I think your reports have covered most of what we need for that.

–Tomasz Czechowski, University of York

Weeping lovegrass

(Eragrostis curvula)

Viviana Echenique, CERZOS-CONICET

The use of Dovetail Chicago, Hi-C and Hi-Rise enabled us to improve the N50 of the first Eragrostis curvula genome assembly from 300 Kb up to 43 Mb. Having almost seven complete chromosomes out of the ten for the species, together with a high level of contiguity (since 83.7% of the genome size was contained in the first 14 scaffolds). This genome assembly have been used successfully to look for genes related to the reproductive mode, drought tolerance and forage quality.

–Viviana Echenique, CERZOS-CONICET

Eggplant

(Solanum melongena)

Yongchao Niu, Biozeron Shenzhen, Inc.

Through the test of our several genome projects, Dovetail’s Hi-C kit has stable and excellent performance in plants, mammals and poultry. By using Dovetail Hi-C and HiRise assembly pipeline, we can obtain high-quality genomes at chromosome level. Taking eggplant as an example, we have obtained the highest quality eggplant genome so far, with contig N50=5.3 Mb and scaffold N50=93.9 Mb. In the future, we will use Dovetail’s Hi-C technology in more genome projects.

–Yongchao Niu, Biozeron Shenzhen, Inc.

Annual bluegrass

(Poa annua)

Christopher Benson, Penn State University

Both the assemblies and the annotations for all species contained >90% conserved orthologs, corroborating their quality. We demonstrate that the parental diploid genomes accurately represent the A and B subgenomes of Poa annua and characterize genetic exchange between and within the subgenomes of Poa annua. We plan to incorporate genomic and transcriptomic resources to aid in better
targeting of Poa annua in turfgrass beeding applications.

–Christopher Benson, Penn State University

Moringa

(Moringa oleifera)

Allen Van Deynze, University of California, Davis

A high quality chromosome-level genome was generated using nanopore and scaffolded using Dovetail Omni-C proximity ligation, critical to development of this crop, enabling hypothesis-driven breeding.

–Allen Van Deynze, University of California, Davis

Coffee

(Coffea arabica)

Susan Strickler, Boyce Thompson Institute

Dovetail did their magic and got back to us with some really improved results. 87% of the assembly is containd within 11 chromosomes. This is very helpful for us because we want to do comparitive genomics and this will help us to do that.

–Susan Strickler, Boyce Thompson Institute

Tomato

(Solanum habrochaites)

Marc Galland, University of Amsterdam

Based on a previously assembled genome sequence of a wild tomato relative Solanum habrochaites PI127826 using Dovetails Genomics technology helped us to obtain a chromosome-level assembly for most of our favorite wild tomato relative Solanum habrochaites Pi127826. Dovetails technology was nicely able to complement previous available 10X Genomics and Optical Mapping information yielding not only an improved assembly but also a genome annotation. Altogether, this new reference genome serves as a new foundation for our attempt to understand the basis of relevant traits into cultivated tomato.

–Marc Galland, University of Amsterdam

Wheat

(Triticum)

Michael Abrouk, KAUST

Our work with Dovetail Genomics has allowed us to generate chromosome-scale assemblies of the large and repeat rich bread-wheat genome (~15 Gbp, >85% TE), which facilitated the discovery of genes that control agriculturally important traits.

–Michael Abrouk, KAUST

Wheat

(Triticum)

Surbhi Grewal, University of Nottingham

The team at Dovetail Genomics was fantastic from the very beginning. They have worked with me to understand and support all the project requirements. The most impressive part was the great communication at all stages and super-fast delivery of data. I really appreciate their efforts and look forward to working with them again in the future.

–Surbhi Grewal, University of Nottingham

Water hyacinth

(Eichhornia paniculata)

Haoran Xue, University of Toronto

Dovetail did an amazing job assembling the genome of annual water hyacinth (Eichhornia paniculata) with PacBio HiFi and Omni-C sequences. We got a chromosome level assembly with a very high BUSCO score (97.2%). We are using it for whole genome alignments to look for the structural variations that make up the tristyly supergenes.

–Haoran Xue, University of Toronto

Japanese stiltgrass

(Microstegium vimineum)

Craig Barrett, West Virginia University

We are extremely happy with Dovetail’s Omni-C genome assembly of Japanese stiltgrass, now published in Genome Biology and Evolution: https://academic.oup.com/gbe/article/13/11/evab238/6413638. Invasive plant species are underrepresented among high-quality genomes, and we now have an invaluable resource for studying one of North America’s most damaging invasives. This has been a critical tool in our studies of trait variation, genomics, adaptation, and aspects of invasion biology that otherwise would have been impossible.

–Craig Barrett, West Virginia University

European ash

(Fraxinus excelsior)

Jarek Burczyk, Kazimierz Wielki University

We have enjoyed collaborating with Dovetail on the Assembly of European ash (many thanks Jasmine and the team). Hi-C scaffolding of Dovetail delivered us an excellent assembly of the 808 Mb ash genome with 98.5% of the assembly located in expected 23 chromosomes. We already placed the new assembly at NCBI (GCA_019097785.1) hoping that it will be an important genomic resource for studying backgrounds for ash resistance against Hymenoscyphus fraxineus, the pathogen causing severe ash dieback across Europe.

–Jarek Burczyk, Kazimierz Wielki University

Green alga

(Zygnema circumcarinatum)

Yanbin Yin, University of Nebraska

Super happy to see the first chromosome-level assembly of a Zygnematophyceae green algal (ZGA) genome (Zygnema circumcarinatum strains SAG 698-1b) with Nanopore long read sequencing + Dovetail HiRise scaffolding. Such a small genomes (70 Mb) with 20 tiny chromosomes! This will keep us (http://bcb.unl.edu/) busy to fully analyze it against other ZGA genomes.

–Yanbin Yin, University of Nebraska

Giant sequoia

(Sequoiadendron giganteum)

David Neale, University of California, Davis

The Hi-C scaffolding Dovetail delivered for our Giant sequoia genome assembly led to the highest quality assembly for any of the large conifer genome assemblies to date. We are using Dovetail’s technology to assemble the hexaploid Coast redwood genome now and will be a part of our standard recipe going forward for all conifer and other tree reference genome sequences.

–David Neale, University of California, Davis

Coast redwood

(Sequoia sempervirens)

Steven Salzberg, Johns Hopkins University

Wow! This is a jaw-droppingly huge improvement! Instead of 474 Kb, we have scaffolds over 61 Mb – that’s over 100 times bigger! Amazing – thanks for this very good news.

–Steven Salzberg, Johns Hopkins University

If you’d like to share your own experience, please contact: MDaly@cantatabio.com.

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Animals

Plants

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