Breaking the Hi-C Resolution Barrier: Micro-C enables chromatin conformation capture at the nucleosome level
Key discussion points:
- How the use of MNase in Micro-C assays enables the highest resolution in chromatin conformation
- Why Micro-C has increased signal-to-noise ratio and what this means for reducing the sequencing burden of conformation studies
- The types of features Micro-C captures that other Hi-C approaches have difficulty detecting, including enhancer-promoter and promoter-promoter interactions
- How Micro-C can be leveraged in protein-direct approaches to chromatin conformation capture assays