- Sequence independent chromatin fragmentation enables even genome-wide detection of chromatin contacts (up to 20% of the genome lacks coverage using restriction enzyme based Hi-C approaches)
- Ultra-high nucleosome-level resolution of chromatin contacts
- Highest signal-to-noise data with both enrichment of long-range informative reads and nucleosome protected fragments
- QC Report
- Sequence data (fastq file format)
- Contact matrices (mcool and hic file formats)
Dovetail™ Micro-C Libraries Enrich For Desirable Hi-C Properties MNase enzyme possesses both sequence-independent endonuclease and exonuclease activities, thereby generating nucleosome length (146 bp) fragments, while the proximity ligation portion of the protocol is optimized to maximize long-range interactions. The resulting highly uniform, short fragments enable nucleosome-level resolution of chromatin contacts, a theoretical resolution maximum.
Improved calling of topological features The ability to detect higher-order features – such as chromatin loops – in proximity ligation data is dependent on enriching long-range, informative reads to capture chromatin interaction frequency. The increased number of chromosome conformation-informative reads, combined with ultra-high-resolution, improves loop calling as compared to RE-based methods.
Dovetail™ Micro-C uniquely captures nucleosome positioning Chromatin digested with MNase reveals a genome-wide nucleosome map that is visible in the Dovetail™ Micro-C libraries. The map consists of sequence read peaks correlating to DNA that is protected by the nucleosome and troughs representing intervening DNA that is accessible to MNase digestion. This oscillation occurs at a frequency of ~146 bp (the length of DNA wrapped around a mono-nucleosome) and is a feature unique to Micro-C data, but absent from RE-based approaches. The combined genome-wide nucleosome positioning and ultra-resolution chromosome topology enabled by Dovetail™ Micro-C facilitates mapping from nucleosome-to-nucleosome chromatin contacts.