The Dovetail Micro-C Kit enables capture of chromatin topology at a mono-nucleosome resolution. The proximity ligation workflow uses micrococcal nuclease (MNase) for even and highly reproducible chromatin fragmentation with the following benefits:
Highest resolution achievable – Capture topological features down to mono-nucleosome positioning
Less cost per sample – Create high resolution contacts maps with lower sequencing depth
Uniform sequence coverage – no restriction site coverage biases due to use of a sequence-independent MNase enzyme
Dovetail Micro-C Technology represents the next evolution to Hi-C for high-resolution mapping of chromatin topological features.
A short 4.5-minute video and slide deck on the Dovetail™ Micro-C approach, how it works and its benefits:
For identical sequencing depths, the number of supporting reads for known loops are significantly higher for Dovetail Micro-C compared to standard restriction enzyme-based Hi-C approaches. This improves the calling of topological features and reduces the required read depth leading to sequencing cost savings.
Metagene analysis of relative coverage across ~20,000 high occupancy CTCF regions showing high nucleosome positions either side of the CTCF binding site. Peaks in coverage represent DNA protected by the nucleosome and troughs are DNA accessible to MNase during chromatin fragmentation.