The Dovetail HiChIP MNase Kit combines the benefits of ChIP-seq with the long-range information of
Hi-C, enabling genome-wide mapping of protein-directed topological features. Compared to ChIP-seq, Dovetail HiChIP not only identifies locally-bound sequences, it also captures distal interactions, up to megabases away, mediated by your protein of interest (e.g. promoter/enhancer interactions).
The Dovetail HiChIP MNase Kit uniquely offers the following benefits:
- Use of MNase unveils topological features down to mono-nucleosome level, enabling the highest resolution mapping of chromatin interactions
- Simple and reproducible workflow – no sonication required
- Deeper understanding of protein function – capture ChIP-seq and Hi-C data in a single library
For ease of adoption, Dovetail has validated 8 commonly-used antibodies, with more on the way. The Dovetail HiChIP MNase Kit is the first commercially available product for analyzing protein-directed chromatin architecture.
A short 4-minute video on the Dovetail HiChIP MNase kit — how it works and its benefits:
Accompanying 5-page Slide Deck:
Dovetail™ HiChIP MNase Tech Note:
Dovetail™ Micro-C Kits
The Micro-C Kit enables capture of chromatin topology at a mono-nucleosome resolution:
- Capture topological features down to mono-nucleosome positioning
- Create high resolution contacts maps with lower sequencing depth
- No restriction site coverage biases due to use of a sequence-independent MNase enzyme
The proximity ligation workflow uses micrococcal nuclease (MNase) for even and highly reproducible chromatin fragmentation.
Dovetail™ Omni-C™ Kits
The Omni-C™ Kit enables NGS library prep that supports multiple whole-genome sequencing applications:
- Large structural variant detection
- SNP & InDel genotyping
- Perform genome assembly
- Perform haplotype phasing
- Capture chromatin conformation data
With a single library prep, you can capture epigenetic and 3-D genome architectural signals while preserving all the applications and capabilities of whole-genome shotgun sequencing data.