From benchtop to terminal: Integrating HiChIP into your workflow

Key discussion points:

  • What is HiChIP
  • What makes MNase-HiChIP different from other HiChIP approaches
  • Wet lab process
    • Method Overview
    • QC metrics and how they are informative
  • Data processing with HiChIP
  • What is a Valid Read Pair without restriction sites?
  • How to use a simple workflow to assess HiChIP data quality
  • How to check chromatin IP enrichment
  • Aligning tips and tricks
  • Interpreting the key informatic metrics