Dovetail™ HiChIP MNase Service Project

Catalogue #: 20017

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Specifications

Delivery Time

10 weeks

Library

Dovetail™ Hi-C

Sequencing Platform

Illumina

Analysis Platform

Dovetail™ QC pipeline

Available Add-On

ChIP-seq MNase Service

Service Description

Dovetail™ HiChIP combines ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Let Dovetail™ scientists take on your HiCHIP project, from sample to sequence data (200M read-pairs per sample) and contact matrices ready for downstream analysis and interpretation. For cells and tissue derived from human or mouse, choose from one of our three antibodies – CTCF, H3K4me3, and H3K27 (IgG is also available as a negative control) – to map protein-directed chromatin conformation. You provide the sample(s), we’ll get you the data.

  • Capture ChIP-seq and Hi-C data together in a single library
  • Choose one of three chromatin related antibodies: CTCF, H3K4me3, or H3K27
  • Map chromatin interactions at nucleosome level resolution
  • QC Report
  • Sequence data (fastq file format)
  • Contact matrices (mcool and hic file formats)

Map protein binding sites and long-range protein-directed interactions    The Dovetail™ HiChIP Kit enables detection of protein-directed 3-D chromatin interactions. Akin to ChIP-seq, the Dovetail™ HiChIP data captures sequences directly bound by the protein of interest. However, additional long-range interactions mediated by the protein of interest are also captured. The use of MNase in the assay results in nucleosome resolution data.

Produce high resolution contact maps with less sequencing    Enrichment of protein-directed chromatin features enables high-resolution contact map generation with less read depth. Compared to a high resolution restriction enzyme-based Hi-C, Dovetail™ HiChIP data enables visualization of higher-order chromatin features, such as loops and chromatin interactions, at a fraction of the read depth leading to significant sequencing costs savings.

Designed to augment a Dovetail™ HiChIP MNase service project, the Dovetail™ ChIP-seq MNase service uses the same chromatin fragment strategy, ensuring the highest data concordance. A Dovetail™ ChIP-seq MNase project is only available with the purchase of Dovetail™ HiChIP MNase service project. Choose from one of our three antibodies – CTCF, H3K4me3, or H3K27 (IgG is also available as a negative control).

Materials & Deliverables

Human or mouse cells, tissue, or blood

  • QC Report
  • Sequence data (fastq file format)
  • Contact matrices (mcool and hic file formats)

Documents

Software

  • Dovetail™ QC Pipeline

Scientific Literature

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