In addition to contiguity improvement, Chicago® data serves another very important function. HiRise™ uses Chicago® data to find and correct contig order and orientation errors in the input assembly. High confidence misjoins are corrected by HiRise™, thereby improving the overall accuracy of the final assembly. In this example, a false join (circled) is clearly evident in the Chicago® plot, but not visible in the Dovetail™ Hi-C plot. In other words, Dovetail™ Hi-C data does not have the resolution to reveal contig order and orientation errors.