- Dovetail™ library construction and sequencing with a specialized combination of our proprietary Dovetail™ Omni-C™, Chicago™ and/or Dovetail™ Hi-C
- Assembly informatics through scaffolding with our cutting-edge assembly pipeline and genome TAD analysis, if applicable
- Tailored approach to scaffolding using proven methodologies
- Designated Scientific Project Manager to answer questions and provide project guidance
- Usable genome assembly within 10 weeks
- Highly accurate results that have been validated by multiple technology types
- Access to Dovetail Genomics proprietary service offerings and expertise
Chicago™ Libraries use a combination of in vitro chromatin fixation, digestion and crosslink reversal to create a library type unique to Dovetail Genomics. This library type has been shown to improve ordering, orientation and contiguity even in highly accurate assemblies.
Dovetail™ Hi-C libraries use a single restriction enzyme (DpnII) for chromatin digestion prior to proximity ligation. This library uses proven Hi-C chemistry well accepted in the genome assembly and chromatin conformation research fields.
Dovetail™ Omni-C™ Technology incorporates a sequence-independent endonuclease approach to chromatin fragmentation in the proximity ligation protocol.
The HiRise™ Scaffolding Pipeline uses the de novo assembly as an input. Multiple iterations are completed layering the use of the library sequencing data to provide a contiguous high-quality assembly.
When available, TAD analysis uses the data collected for the assembly creation to provide a view into chromatin 3D architecture, including topologically associated domains.