Four steps to check your kit-generated data quality
With the assay completed and your library in hand, you are now ready to assess the quality of your library. The following four steps enable assessment of library quality.
Step 1: Sequence the library
Sequence the library using paired-end sequencing (2 x 75 bp, 2 x 100 bp, or 2 x 150 bp). Given the Dovetail computational QC process requirements, you’ll need to sequence between 1 and 2 million total read pairs. Whether you perform sequence yourself or submit to a service provider, your Omni-C or Micro-C libraries can be treated like any other next generation sequencing library of high diversity. No special sequencing considerations are required.